Hellberg P O, Källskog T O
Department of Physiology and Medical Biophysics, University of Uppsala, Sweden.
Kidney Int. 1989 Oct;36(4):555-61. doi: 10.1038/ki.1989.230.
Neutropenia was induced in male Sprague-Dawley rats by administration of antineutrophil serum (ANS). A control group received an equal volume of inactive serum. After 45 minutes of unilateral complete renal ischemia the renal blood flow (RBF) was measured by an electromagnetic flow meter. The net filtration force (NFF) in glomerular capillaries, single nephron filtration rate (SNGFR) and frequency of tubular obstructions were estimated by a micropuncture technique. Tubular leakage was measured from the fractional recovery in the normal contralateral kidney of 3H- or 14C-inulin injected into surface proximal and distal tubules of the post-ischemic kidney. Neither ANS nor inactive serum had any influence on inulin clearance (CIn) in the normal kidney. In the post-ischemic kidney, CIn was four times higher in ANS-treated than in control animals. There was no difference in RBF, NFF, SNGFR or the frequency of tubular obstructions between neutrophil-depleted and control animals. The transtubular leakage of inulin injected into proximal tubules was substantially less in the ANS-treated than in the control group (11.3 +/- 1.5% vs. 35.1 +/- 6.5%; P less than 0.01). But distal tubular leakage was equal in the two groups. The control group showed isothenuria (350 +/- 29 mOsm.kg-1), while ANS-treated animals produced hyperosmolar urine (555 +/- 60 mOsm.kg-1; P less than 0.05). It is concluded that neutrophil granulocytes mediate post-ischemic tubular leakage, which contributes to the depression in renal clearance parameters and the inability to produce hyperosmolar urine.
通过给予抗中性粒细胞血清（ANS）在雄性斯普拉格-道利大鼠中诱导中性粒细胞减少。对照组接受等体积的无活性血清。在单侧完全肾缺血45分钟后，用电磁流量计测量肾血流量（RBF）。通过微穿刺技术估计肾小球毛细血管中的净滤过力（NFF）、单个肾单位滤过率（SNGFR）和肾小管阻塞频率。从注入缺血后肾脏表面近端和远端小管的3H-或14C-菊粉在正常对侧肾脏中的分数回收率来测量肾小管渗漏。ANS和无活性血清对正常肾脏中的菊粉清除率（CIn）均无影响。在缺血后肾脏中，ANS处理组的CIn比对照组动物高四倍。中性粒细胞减少组和对照组动物在RBF、NFF、SNGFR或肾小管阻塞频率方面没有差异。注入近端小管的菊粉的跨小管渗漏在ANS处理组中比对照组明显更少（11.3±1.5%对35.1±6.5%；P<0.01）。但两组的远端小管渗漏相等。对照组表现为等渗尿（350±29 mOsm.kg-1），而ANS处理的动物产生高渗尿（555±60 mOsm.kg-1；P<0.05）。结论是中性粒细胞介导缺血后肾小管渗漏，这导致肾清除参数降低和无法产生高渗尿。
