Xie Yong, Zhang Lei, Yang Xian, Le Tao
a College of Life Science , Chongqing Normal University , Chongqing , China.
b Department of Animation , Chongqing College of Electronic Engineering , Chongqing , China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017 Mar;34(3):371-378. doi: 10.1080/19440049.2016.1277038. Epub 2017 Jan 23.
A rapid and sensitive immunochromatographic test strip (ICTS) was developed in a competitive format with the quantum dot-conjugated monoclonal antibody specifically to determine the residues of oxytetracycline (OTC) and its metabolite 4-epi-oxytetracycline (4-epi-OTC). Using an ICTS reader, the 50% inhibition concentration (IC) was found to be 3.41 ± 0.29 ng ml of OTC in phosphate-buffered saline samples and the detection limit was 0.44 ± 0.05 ng ml. The visual cut-off level of the ICTS is 25 ng ml. The recoveries from tissue samples spiked with OTC of 50-600 μg kg were 74.2-107.2%, with coefficients of variation below 15%. The method was used to analyse incurred tissue samples, and there was good correlation (R = 0.999) between the ICTS and high-performance liquid chromatography. These results indicate that ICTS is suitable for the rapid and quantitative detection of OTC and 4-epi-OTC residues in edible animal tissues.
开发了一种快速灵敏的免疫层析试纸条（ICTS），采用竞争法，将量子点偶联单克隆抗体用于特异性测定土霉素（OTC）及其代谢物4-表土霉素（4-epi-OTC）的残留量。使用ICTS读数仪，发现磷酸盐缓冲盐水样品中OTC的50%抑制浓度（IC）为3.41±0.29 ng/ml，检测限为0.44±0.05 ng/ml。ICTS的目视截断水平为25 ng/ml。在添加了50 - 600μg/kg OTC的组织样品中的回收率为74.2 - 107.2%，变异系数低于15%。该方法用于分析实际检测的组织样品，ICTS与高效液相色谱法之间具有良好的相关性（R = 0.999）。这些结果表明，ICTS适用于快速定量检测食用动物组织中土霉素和4-表土霉素的残留量。
