用于快速检测和区分猪丹毒丝菌疫苗株和野生型菌株的双重PCR方法的建立。
Development of a duplex PCR for rapid detection and differentiation of Erysipelothrix rhusiopathiae vaccine strains and wild type strains.
作者信息
Zhu Weifeng, Wu Chao, Kang Chao, Cai Chengzhi, Jin Meilin
机构信息
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China,; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, China; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
出版信息
Vet Microbiol. 2017 Feb;199:108-110. doi: 10.1016/j.vetmic.2016.11.016. Epub 2016 Nov 17.
The differentiation of vaccine strains from wild type strains is important for disease control. A duplex PCR for rapid detection and differentiation of Erysipelothrix rhusiopathiae vaccine strains and wild type strains was developed based on the DNA polymerase IV gene. This duplex PCR was sensitive and specific. The detection results were coincident with that of a single nucleotide polymorphisms based PCR but the detection process was more rapid. In conclusion, this duplex PCR was a useful tool for Erysipelothrix rhusiopathiae infections' differential diagnosis in China.
区分疫苗株和野生型菌株对于疾病控制至关重要。基于DNA聚合酶IV基因，开发了一种用于快速检测和区分猪丹毒杆菌疫苗株和野生型菌株的双重PCR方法。该双重PCR具有敏感性和特异性。检测结果与基于单核苷酸多态性的PCR结果一致，但检测过程更快。总之，这种双重PCR是中国猪丹毒杆菌感染鉴别诊断的有用工具。
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