β-肾上腺素能拮抗剂普萘洛尔可抑制克氏锥虫循环后期形态对哺乳动物细胞溶酶体的扩散和侵袭。

Beta-adrenergic antagonist propranolol inhibits mammalian cell lysosome spreading and invasion by Trypanosoma cruzi metacyclic forms.

作者信息

Macedo Silene, Rodrigues João Paulo Ferreira, Schenkman Sergio, Yoshida Nobuko

机构信息

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, R. Pedro de Toledo, 669 - 6(o) andar, 04039-032, São Paulo, SP, Brazil.

出版信息

Microbes Infect. 2017 Apr-May;19(4-5):295-301. doi: 10.1016/j.micinf.2017.01.004. Epub 2017 Jan 19.

DOI:10.1016/j.micinf.2017.01.004

PMID:28111357

Abstract

The involvement of β-adrenergic receptor (β-AR) in host cell invasion by Trypanosoma cruzi metacyclic trypomastigote (MT) is not known. We examined whether isoproterenol, an agonist of β-AR, or nonselective β-blocker propranolol affected MT internalization mediated the stage-specific surface molecule gp82. Treatment of HeLa cells with propranolol significantly inhibited MT invasion whereas isoproterenol had no effect. Propranolol, but not isoproterenol, also inhibited the lysosome spreading required for gp82-dependent MT invasion. The effect of propranolol in inhibiting MT internalization was not due to the prevention of gp82 interaction with β-AR. It was mainly associated with its ability to impair lysosome spreading.

摘要

β-肾上腺素能受体（β-AR）是否参与克氏锥虫循环后期锥鞭毛体（MT）对宿主细胞的入侵尚不清楚。我们研究了β-AR激动剂异丙肾上腺素或非选择性β-阻滞剂普萘洛尔是否会影响由阶段特异性表面分子gp82介导的MT内化。用普萘洛尔处理HeLa细胞可显著抑制MT入侵，而异丙肾上腺素则无此作用。普萘洛尔而非异丙肾上腺素，也抑制了gp82依赖性MT入侵所需的溶酶体扩散。普萘洛尔抑制MT内化的作用并非由于阻止gp82与β-AR相互作用。它主要与其损害溶酶体扩散的能力有关。