定位候选基因谷氨酸受体相互作用蛋白1中的错义突变与猪背膘厚度性状的关联
Association of a missense mutation in the positional candidate gene glutamate receptor-interacting protein 1 with backfat thickness traits in pigs.
作者信息
Lee Jae-Bong, Park Hee-Bok, Yoo Chae-Kyoung, Kim Hee-Sung, Cho In-Cheol, Lim Hyun-Tae
机构信息
Korea Zoonosis Research Institute (KoZRI), Chonbuk National University, Iksan 54531, Korea.
Subtropical Livestock Research Institute, National Institute of Animal Science, Jeju 63242, Korea.
出版信息
Asian-Australas J Anim Sci. 2017 Aug;30(8):1081-1085. doi: 10.5713/ajas.16.0414. Epub 2016 Dec 27.
OBJECTIVE
Previously, we reported quantitative trait loci (QTLs) affecting backfat thickness (BFT) traits on pig chromosome 5 () in an F2 intercross population between Landrace and Korean native pigs. The aim of this study was to evaluate glutamate receptor-interacting protein 1 () as a positional candidate gene underlying the QTL affecting BFT traits.
METHODS
Genotype and phenotype analyses were performed using the 1,105 F progeny. A mixed-effect linear model was used to access association between these single nucleotide polymorphism (SNP) markers and the BFT traits in the F intercross population.
RESULTS
Highly significant associations of two informative SNPs (c.2442 T>C, c.3316 C>G [R1106G]) in with BFT traits were detected. In addition, the two SNPs were used to construct haplotypes that were also highly associated with the BFT traits.
CONCLUSION
The SNPs and haplotypes of the gene determined in this study can contribute to understand the genetic structure of BFT traits in pigs.
目的
此前，我们报道了在长白猪和韩国本土猪的F2杂交群体中，影响猪5号染色体（）背膘厚度（BFT）性状的数量性状基因座（QTL）。本研究的目的是评估谷氨酸受体相互作用蛋白1（）作为影响BFT性状的QTL潜在的位置候选基因。
方法
对1105只F代后代进行基因型和表型分析。采用混合效应线性模型来研究这些单核苷酸多态性（SNP）标记与F杂交群体中BFT性状之间的关联。
结果
检测到基因中两个信息性SNP（c.2442 T>C，c.3316 C>G [R1106G]）与BFT性状高度显著相关。此外，这两个SNP用于构建单倍型，其也与BFT性状高度相关。
结论
本研究确定的基因的SNP和单倍型有助于了解猪BFT性状的遗传结构。
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