Omotehara Takuya, Minami Kiichi, Mantani Youhei, Umemura Yuria, Nishida Miho, Hirano Tetsushi, Yoshioka Hidefumi, Kitagawa Hiroshi, Yokoyama Toshifumi, Hoshi Nobuhiko
Laboratory of Molecular Morphology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
Laboratory of Histophysiology, Department of Animal Science, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
Dev Dyn. 2017 Feb;246(2):148-156. doi: 10.1002/dvdy.24469.
The left male gonad in the chicken embryo has a thickened cortical layer, but it eventually becomes flattened after the onset of testicular development. Because the destination of the cortical cells migrating from the left gonad remains unclear, we examined this issue herein.
The testis-inducing gene doublesex- and mab-3-related transcription factor 1 (DMRT1) was detected in a proportion of the columnar and cubic epithelial cells in the cortex of the left testis as well as Sertoli cells in both testes. Interestingly, some of the DMRT1-expressing cortical cells were contiguous with Sertoli cells in the testis cord. Some cortical cells exhibited a vimentin-positive cytoplasm that was elongated all the way to the medulla. In addition, a desmosome-like structure was observed between the elongated cytoplasm in these cells and the adjacent Sertoli cell. After the organ culture, a few cells labeled with a fluorescent dye that stained only the cortical cells at the beginning of the culture were located in the testis cord of the left testis.
Some cortical cells expressing DMRT1 were suggested to contribute to the Sertoli cells in the testis cord only after the onset of testicular development and only in the left testis. Developmental Dynamics 246:148-156, 2017. © 2016 Wiley Periodicals, Inc.
鸡胚中的左侧雄性性腺具有增厚的皮质层，但在睾丸发育开始后最终会变平。由于从左侧性腺迁移的皮质细胞的去向尚不清楚，我们在此研究了这个问题。
在左侧睾丸皮质的一部分柱状和立方上皮细胞以及两个睾丸的支持细胞中检测到睾丸诱导基因双性和 mab-3 相关转录因子 1（DMRT1）。有趣的是，一些表达 DMRT1 的皮质细胞与睾丸索中的支持细胞相邻。一些皮质细胞表现出波形蛋白阳性的细胞质，一直延伸到髓质。此外，在这些细胞的伸长细胞质与相邻支持细胞之间观察到一种桥粒样结构。器官培养后，少数在培养开始时仅标记皮质细胞的荧光染料标记的细胞位于左侧睾丸的睾丸索中。
一些表达 DMRT1 的皮质细胞仅在睾丸发育开始后且仅在左侧睾丸中才对睾丸索中的支持细胞有贡献。《发育动力学》246:148 - 156，2017 年。©2016 威利期刊公司。
