Beynen A C, Sijtsma S R, Kiepurski A K, West C E, Baumans V, Van Herck H, Stafleu F R, Van Tintelen G
Chair of Laboratory Animal Science, Agricultural University, Wageningen, The Netherlands.
Lab Anim. 1989 Oct;23(4):307-12. doi: 10.1258/002367789780745980.
One day old pullets derived from marginally vitamin A deficient laying hens were fed diets containing either adequate or marginal amounts of vitamin A. At the age of 34 days, animals fed the diet low in vitamin A had group mean plasma concentrations of retinol which were one tenth the mean plasma concentrations of controls. When compared with their controls, the deficient animals displayed body weights which were on average 16% less. Of 20 pullets per dietary group one control animal and 9 deficient animals died by the age of 34 days. At the age of 29 days, control (n = 16) and deficient chickens (n = 11) were examined clinically by assigning scores to a number of parameters. Three assessors carried out the examination independently. The birds were presented for examination at random and their treatment groups were not disclosed to the assessors. Out of 26 parameters assessed quantitatively per individual animal, only three parameters discriminated between control and deficient chickens. Deficient animals grew poorly, had a hunched up posture and increased fluid content in faeces. Classical signs of chronic vitamin A deficiency in domestic fowl such as bone deformities, keratinization of the tongue and decreased transparency of the cornea were not observed.
从维生素A轻度缺乏的产蛋母鸡所产的一日龄小母鸡，被喂食含有充足或微量维生素A的日粮。在34日龄时，喂食低维生素A日粮的动物组平均血浆视黄醇浓度仅为对照组平均血浆浓度的十分之一。与对照组相比，缺乏维生素A的动物体重平均轻16%。每个日粮组的20只小母鸡中，到34日龄时，有1只对照动物和9只缺乏维生素A的动物死亡。在29日龄时，对对照组（n = 16）和缺乏维生素A组（n = 11）的鸡进行了临床检查，通过对多个参数打分来评估。三名评估人员独立进行检查。将鸡随机送来检查，评估人员不知道它们的处理组情况。在每只动物定量评估的26个参数中，只有三个参数能区分对照组和缺乏维生素A组的鸡。缺乏维生素A的动物生长不良，呈蜷缩姿势，粪便含水量增加。在家禽中未观察到慢性维生素A缺乏的典型症状，如骨骼畸形、舌角化和角膜透明度降低。
