Simultaneous determination of lactate, pyruvate, and ascorbate in microdialysis samples from rat brain, blood, fat, and muscle using high-performance liquid chromatography.
作者信息
Hallström A, Carlsson A, Hillered L, Ungerstedt U
机构信息
Department of Pharmacology, Karolinska Institutet, Carnegie Medicin AB, Stockholm, Sweden.
出版信息
J Pharmacol Methods. 1989 Sep;22(2):113-24. doi: 10.1016/0160-5402(89)90040-5.
PMID:2811388
Abstract
An isocratic high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of lactate, pyruvate, and ascorbate in tissue and blood, sampled in vivo using the microdialysis technique. The compounds were separated on a silica-based, reversed-phase column or on a resin-based, cation exchange column. The acids were monitored by ultraviolet detection.
摘要
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