Matsumura Nozomi, Goto Satoshi, Uchio Eiichi, Nakajima Kyoko, Fujita Takeshi, Kadonosono Kazuaki
Department of Ophthalmology, Kanagawa Children's Medical Center, Yokohama, Japan.
Department of Ophthalmology, The Jikei University School of Medicine, Tokyo, Japan.
Invest Ophthalmol Vis Sci. 2017 Jan 1;58(1):252-256. doi: 10.1167/iovs.16-20717.
This study evaluated the cytokine levels in unilateral tear samples from both sides in patients with pediatric lacrimal duct obstruction.
Fifteen cases of unilateral lacrimal duct obstruction (mean, 26.9 ± 28.7 months old) were enrolled in this study. Tear samples were collected separately from the obstructed side and the intact side in each case before surgery, which was performed under general anesthesia or sedation. The levels of IL-2, IL-4, IL-6, IL-10, TNF, IFN-γ, and IL-17A then were measured in each tear sample. A receiver operating characteristic (ROC) curve was constructed for the IL-6 levels in the tears. We also measured the postoperative tear fluid levels of IL-6 in those cases from which tear fluid samples could be collected after the surgery.
Only the IL-6 concentration was significantly higher on the lacrimal duct-obstructed sides, compared to the control sides (P < 0.001). An ROC curve analysis for the IL-6 levels in tears showed a high value for discriminating the lacrimal duct-obstructed side from the control side (area under the ROC curve [AUC], 0.99; 95% confidence interval [CI], 0.968-1). Significant decrease of the tear fluid IL-6 levels was observed in the seven cases from which tear fluid samples also could be collected after the surgery (P = 0.016).
The IL-6 level in tear fluid was significantly higher on the sides with lacrimal duct obstruction, compared to the control sides, and could be a biomarker for pediatric lacrimal duct obstruction.
本研究评估了小儿泪道阻塞患者双侧单侧泪液样本中的细胞因子水平。
本研究纳入了15例单侧泪道阻塞患者（平均年龄26.9±28.7个月）。在全身麻醉或镇静下手术前，分别从每例患者的阻塞侧和未阻塞侧采集泪液样本。然后测量每个泪液样本中白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、肿瘤坏死因子（TNF）、干扰素-γ（IFN-γ）和白细胞介素-17A的水平。构建泪液中IL-6水平的受试者工作特征（ROC）曲线。我们还测量了术后能采集到泪液样本的那些病例的泪液中IL-6的水平。
与对照侧相比，仅泪道阻塞侧的IL-6浓度显著更高（P<0.001）。泪液中IL-6水平的ROC曲线分析显示，区分泪道阻塞侧和对照侧的值较高（ROC曲线下面积[AUC]为0.99；95%置信区间[CI]为0.968 - 1）。在术后也能采集到泪液样本的7例患者中，观察到泪液IL-6水平显著降低（P = 0.016）。
与对照侧相比，泪道阻塞侧泪液中的IL-6水平显著更高，并且可能是小儿泪道阻塞 的生物标志物。
