Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
J Environ Sci (China). 2017 Jan;51:181-190. doi: 10.1016/j.jes.2016.06.026. Epub 2016 Aug 26.
To evaluate the protective effects of Glycyrrhiza polysaccharide (GPS) against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced hepatotoxicity in Jian carp, the fish were fed diets containing GPS at doses of 0.1, 0.5 and 1.0g/kg for 60days before an intraperitoneal injection of 0.6μg/kg TCDD at a volume of 0.05mL/10g body weight. At 72hr post-injection, blood and liver samples were taken for biochemical analysis and the fish liver samples were used for the preparation of pathological slices. The results showed that increases in alanine aminotransferase (GPT), aspartate aminotransferase (GOT), lactate dehydrogenase (LDH), and alkaline phosphatase (AKP) in serum induced by TCDD were significantly inhibited by pre-treatment with 1.0g/kg GPS. Following the 1.0g/kg GPS pre-treatment, total protein (TP), albumin (Alb), catalase (CAT), glutathione peroxidase (GPx), total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activities in liver tissue increased significantly, malondialdehyde (MDA) formation (P<0.05 or P<0.01) was significantly inhibited, and the expression of cytochrome P4501A (CYP1A), aryl hydrocarbon receptor 2 (AHR2) and aryl hydrocarbon receptor nuclear translocator 2 (ARNT2) mRNA (P<0.05) was significantly enhanced. Histological observations on fish liver were obtained by preparing paraffin tissue sections via HE staining, and the results showed that histological changes were obviously reduced by 0.5 and 1.0g/kg GPS. GPS significantly reduced liver tissue damage caused by TCDD. Overall, these results proved the hepatoprotective effect of GPS in protecting against fish liver injury induced by TCDD, and supported the use of GPS (1.0g/kg) as a hepatoprotective and antioxidant agent in fish.
为了评估甘草多糖（GPS）对 2,3,7,8-四氯二苯并对二恶英（TCDD）诱导的建鲤肝毒性的保护作用，将鱼用含有 0.1、0.5 和 1.0g/kg GPS 的饲料喂养 60 天，然后在腹腔内注射 0.6μg/kg TCDD（体积为 0.05mL/10g 体重）。注射后 72 小时，采集血液和肝脏样本进行生化分析，并制备鱼肝脏病理切片。结果表明，TCDD 诱导的血清丙氨酸氨基转移酶（GPT）、天冬氨酸氨基转移酶（GOT）、乳酸脱氢酶（LDH）和碱性磷酸酶（AKP）升高，经 1.0g/kg GPS 预处理后明显受到抑制。经 1.0g/kg GPS 预处理后，肝脏组织中总蛋白（TP）、白蛋白（Alb）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、总抗氧化能力（T-AOC）和超氧化物歧化酶（SOD）活性明显升高，丙二醛（MDA）形成明显抑制（P<0.05 或 P<0.01），细胞色素 P4501A（CYP1A）、芳烃受体 2（AHR2）和芳烃受体核转位蛋白 2（ARNT2）mRNA 表达明显增强（P<0.05）。通过 HE 染色制备石蜡组织切片对鱼肝脏进行组织学观察，结果表明，0.5 和 1.0g/kg GPS 明显减少了肝组织损伤。GPS 明显减轻了 TCDD 引起的肝组织损伤。总的来说，这些结果证明了 GPS 在保护 TCDD 诱导的鱼肝损伤方面的保肝作用，并支持将 GPS（1.0g/kg）作为鱼类的保肝和抗氧化剂使用。
