Guo Wen-ya, Cui Yan-mei, Wang Ting-ting, Yu De-yue, Huang Fang
State Key Laboratory of Crop Genetics & Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan. 2017 Jan 20;39(1):56-65. doi: 10.16288/j.yczz.16-144.
LEAFY/FLORICAULA (LFY/FLO) is a family of plant-specific transcription factors, which plays an important role(s) in the regulation of floral organ formation and development. So far, LFY regulation on floral development in wild soybean has not been reported in the literature. In this study, the LFY gene, GsLFY, has been isolated from Glycine soja, and characterized with molecular and transgenic techniques. The cDNA for GsLFY gene is 1224 bp in length and contains an open reading frame encoding a polypeptide of 407 amino acids. Quantitative RT-PCR analysis shows that GsLFY is prominently expressed in various tissues, including roots, flowers and seeds. Among the four floral organs, GsLFY is expressed highly in sepals and stamens while weakly in the petals and carpels. Yeast two-hybrid experiments show that GsLFY possesses transactivation activity while transient expression analysis with Arabidopsis thaliana protoplasts shows that GsLFY protein is localized in the nucleus, supporting the notion that GsLFY is a transcription factor. The GsLFY transgenic tobacco plants flower about 29 days earlier than the wild-type plants, thereby providing a potential rationale for developing new soybean varieties with altered flowering time.
叶状/花椰菜（LEAFY/FLORICAULA，LFY/FLO）是一类植物特有的转录因子家族，在花器官形成和发育的调控中发挥着重要作用。迄今为止，关于野生大豆中LFY对花发育的调控，尚未见文献报道。在本研究中，从野生大豆中分离出了LFY基因GsLFY，并采用分子和转基因技术对其进行了鉴定。GsLFY基因的cDNA长度为1224 bp，包含一个编码407个氨基酸多肽的开放阅读框。定量RT-PCR分析表明，GsLFY在包括根、花和种子在内的各种组织中均有显著表达。在四种花器官中，GsLFY在萼片和雄蕊中高表达，而在花瓣和心皮中低表达。酵母双杂交实验表明GsLFY具有反式激活活性，而用拟南芥原生质体进行的瞬时表达分析表明GsLFY蛋白定位于细胞核，这支持了GsLFY是一种转录因子的观点。转GsLFY基因的烟草植株比野生型植株提前约29天开花，从而为培育开花时间改变的大豆新品种提供了潜在的理论依据。
