Massi Muhammad Nasrum, Biatko Karya Triko, Handayani Irda, Pratama Muhammad Yogi, Septriani Sari, Nurdin Gaby Maulida, Ali Marina B
Department of Medical Microbiology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
Department of Orthopedic and Traumotology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
J Orthop. 2017 Jan 9;14(1):189-191. doi: 10.1016/j.jor.2016.12.003. eCollection 2017 Mar.
To detect DNA and rifampicin resistance in vertebral bone tissue specimens from spondylitis TB suspects.
The rapid GeneXpert MTB/RIF and MGIT 960 liquid culture methods have been used in the specimens. Results from 70 suspects with spondylitis TB shown that 31.42% identified positive for spondylitis TB using culture method, while 88.57% shown positive results using rapid GeneXpert MTB/RIF method. The validity of GeneXpert MTB/RIF shown sensitivity value of 100%, specificity value of 16.6%, PPV of 35.48%, and NPV of 100%.
GeneXpert has a high sensitivity but low specificity value in this study.
检测疑似脊柱结核患者椎骨组织标本中的DNA及利福平耐药情况。
对标本采用了快速GeneXpert MTB/RIF及MGIT 960液体培养方法。70例疑似脊柱结核患者的结果显示，培养法检测脊柱结核阳性率为31.42%，而快速GeneXpert MTB/RIF法阳性率为88.57%。GeneXpert MTB/RIF的有效性显示敏感性值为100%，特异性值为16.6%，阳性预测值为35.48%，阴性预测值为100%。
在本研究中，GeneXpert敏感性高但特异性低。
