利用疑似脊柱结核患者椎骨的DNA组织标本对GeneXpert MTB/RIF快速检测方法进行评估。
Evaluation of rapid GeneXpert MTB/RIF method using DNA tissue specimens of vertebral bones in patients with suspected spondylitis TB.
作者信息
Massi Muhammad Nasrum, Biatko Karya Triko, Handayani Irda, Pratama Muhammad Yogi, Septriani Sari, Nurdin Gaby Maulida, Ali Marina B
机构信息
Department of Medical Microbiology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
Department of Orthopedic and Traumotology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
出版信息
J Orthop. 2017 Jan 9;14(1):189-191. doi: 10.1016/j.jor.2016.12.003. eCollection 2017 Mar.
Abstract
PURPOSE
To detect DNA and rifampicin resistance in vertebral bone tissue specimens from spondylitis TB suspects.
METHODS AND RESULTS
The rapid GeneXpert MTB/RIF and MGIT 960 liquid culture methods have been used in the specimens. Results from 70 suspects with spondylitis TB shown that 31.42% identified positive for spondylitis TB using culture method, while 88.57% shown positive results using rapid GeneXpert MTB/RIF method. The validity of GeneXpert MTB/RIF shown sensitivity value of 100%, specificity value of 16.6%, PPV of 35.48%, and NPV of 100%.
CONCLUSION
GeneXpert has a high sensitivity but low specificity value in this study.
摘要
目的
检测疑似脊柱结核患者椎骨组织标本中的DNA及利福平耐药情况。
方法与结果
对标本采用了快速GeneXpert MTB/RIF及MGIT 960液体培养方法。70例疑似脊柱结核患者的结果显示，培养法检测脊柱结核阳性率为31.42%，而快速GeneXpert MTB/RIF法阳性率为88.57%。GeneXpert MTB/RIF的有效性显示敏感性值为100%，特异性值为16.6%，阳性预测值为35.48%，阴性预测值为100%。
结论
在本研究中，GeneXpert敏感性高但特异性低。
相似文献
1
Evaluation of rapid GeneXpert MTB/RIF method using DNA tissue specimens of vertebral bones in patients with suspected spondylitis TB.利用疑似脊柱结核患者椎骨的DNA组织标本对GeneXpert MTB/RIF快速检测方法进行评估。
J Orthop. 2017 Jan 9;14(1):189-191. doi: 10.1016/j.jor.2016.12.003. eCollection 2017 Mar.
2
Use of GeneXpert Mycobacterium tuberculosis/rifampicin for rapid detection of rifampicin resistant Mycobacterium tuberculosis strains of clinically suspected multi-drug resistance tuberculosis cases.GeneXpert 结核分枝杆菌/利福平快速检测在临床疑似耐多药结核病病例中检测利福平耐药结核分枝杆菌菌株中的应用。
Ann Transl Med. 2016 May;4(9):168. doi: 10.21037/atm.2016.05.09.
3
Assessment of the Diagnostic Utility of GeneXpert Mycobacterium tuberculosis/Rifampicin (MTB/RIF) Assay in the Suspected Cases of Tuberculous Meningitis.GeneXpert 结核分枝杆菌/利福平（MTB/RIF）检测在疑似结核性脑膜炎病例中的诊断效用评估
Cureus. 2023 Apr 18;15(4):e37761. doi: 10.7759/cureus.37761. eCollection 2023 Apr.
4
Initial experience with GeneXpert MTB/RIF assay in the Arkansas Tuberculosis Control Program.阿肯色州结核病控制项目中对GeneXpert MTB/RIF检测的初步经验。
Australas Med J. 2014 May 31;7(5):203-7. doi: 10.4066/AMJ.2014.1905. eCollection 2014.
5
Evaluation of the GeneXpert MTB/RIF assay on extrapulmonary and respiratory samples other than sputum: a low burden country experience.在痰液以外的肺外和呼吸道样本上评估GeneXpert MTB/RIF检测：一个低负担国家的经验
Pathology. 2017 Jan;49(1):70-74. doi: 10.1016/j.pathol.2016.10.004. Epub 2016 Nov 29.
6
Detection of Mycobacterium tuberculosis in AFB smear-negative sputum specimens through MTB culture and GeneXpert MTB/RIF assay.通过 MTB 培养和 GeneXpert MTB/RIF 检测对 AFB 涂片阴性痰标本中结核分枝杆菌的检测。
Int J Immunopathol Pharmacol. 2019 Jan-Dec;33:2058738419827174. doi: 10.1177/2058738419827174.
7
Evaluation of GeneXpert MTB/RIF for detecting Mycobacterium tuberculosis in a hospital in China.在中国一家医院评估GeneXpert MTB/RIF用于检测结核分枝杆菌的情况。
J Int Med Res. 2017 Apr;45(2):816-822. doi: 10.1177/0300060517698618. Epub 2017 Mar 29.
8
[Evaluation of GeneXpert MTB/RIF and BACTEC-MGIT 960 for the detection of tuberculosis among pneumoconiosis-associated tuberculosis patients].[GeneXpert MTB/RIF和BACTEC-MGIT 960用于尘肺病合并肺结核患者肺结核检测的评估]
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2019 Sep 20;37(9):690-693. doi: 10.3760/cma.j.issn.1001-9391.2019.09.014.
9
Evaluation of GeneXpert MTB/RIF for determination of rifampicin resistance among new tuberculosis cases in west and northwest Iran.评估GeneXpert MTB/RIF用于测定伊朗西部和西北部新结核病病例中的利福平耐药性。
New Microbes New Infect. 2017 Jul 13;19:117-120. doi: 10.1016/j.nmni.2017.07.002. eCollection 2017 Sep.
10
Evaluation of GeneXpert /Rifampin for the detection of complex and rifampicin resistance in nonrespiratory clinical specimens.使用GeneXpert/利福平检测非呼吸道临床标本中的复合耐药和利福平耐药情况评估
Int J Mycobacteriol. 2019 Apr-Jun;8(2):132-137. doi: 10.4103/ijmy.ijmy_83_19.
引用本文的文献
1
Diagnostic accuracy of GeneXpert in the diagnosis of spinal tuberculosis: A systematic review and meta-analysis.GeneXpert在脊柱结核诊断中的诊断准确性：一项系统评价与荟萃分析
Narra J. 2024 Aug;4(2):e925. doi: 10.52225/narra.v4i2.925. Epub 2024 Aug 13.
2
infection of the wrist joint: A current concepts review.腕关节感染：当前概念综述
J Clin Orthop Trauma. 2023 Sep 28;44:102257. doi: 10.1016/j.jcot.2023.102257. eCollection 2023 Sep.
3
Efficacy of Xpert in tuberculosis diagnosis based on various specimens: a systematic review and meta-analysis.基于各种标本的 Xpert 在结核病诊断中的疗效：系统评价和荟萃分析。
Front Cell Infect Microbiol. 2023 May 2;13:1149741. doi: 10.3389/fcimb.2023.1149741. eCollection 2023.
4
Xpert MTB/RIF Ultra and Xpert MTB/RIF assays for extrapulmonary tuberculosis and rifampicin resistance in adults.Xpert MTB/RIF Ultra 检测和 Xpert MTB/RIF 检测在成人肺外结核和利福平耐药检测中的应用。
Cochrane Database Syst Rev. 2021 Jan 15;1(1):CD012768. doi: 10.1002/14651858.CD012768.pub3.
5
Diagnostic accuracy of the Xpert MTB/RIF assay for bone and joint tuberculosis: A meta-analysis.Xpert MTB/RIF assay 对骨与关节结核的诊断准确性：一项荟萃分析。
PLoS One. 2019 Aug 22;14(8):e0221427. doi: 10.1371/journal.pone.0221427. eCollection 2019.
6
Xpert MTB/RIF assay for extrapulmonary tuberculosis and rifampicin resistance.用于肺外结核病和利福平耐药性的Xpert MTB/RIF检测
Cochrane Database Syst Rev. 2018 Aug 27;8(8):CD012768. doi: 10.1002/14651858.CD012768.pub2.
7
Diagnostic accuracy evaluation of the conventional and molecular tests for Spinal Tuberculosis in a cohort, head-to-head study.头对头队列研究评估传统与分子检测方法诊断脊柱结核的准确性。
Emerg Microbes Infect. 2018 Jun 20;7(1):109. doi: 10.1038/s41426-018-0114-1.
8
Diagnostic accuracy of Xpert MTB/RIF assay for musculoskeletal tuberculosis: a meta-analysis.Xpert MTB/RIF检测对肌肉骨骼结核的诊断准确性：一项荟萃分析。
Infect Drug Resist. 2017 Sep 28;10:299-305. doi: 10.2147/IDR.S145843. eCollection 2017.
9
Whole Genome Sequencing of Mycobacterium tuberculosis Isolates From Extrapulmonary Sites.肺外部位结核分枝杆菌分离株的全基因组测序
OMICS. 2017 Jul;21(7):413-425. doi: 10.1089/omi.2017.0070.
本文引用的文献
1
Tuberculous spondylodiscitis: epidemiology, clinical features, treatment, and outcome.结核性脊柱骨髓炎：流行病学、临床特征、治疗和预后。
Eur Rev Med Pharmacol Sci. 2012 Apr;16 Suppl 2:58-72.
2
Can a simple flotation method lower the limit of detection of Mycobacterium tuberculosis in extrapulmonary samples analyzed by the GeneXpert MTB/RIF assay?一种简单的漂浮法能否降低 GeneXpert MTB/RIF 检测系统检测肺外样本中结核分枝杆菌的检测限？
J Clin Microbiol. 2012 Jul;50(7):2272-6. doi: 10.1128/JCM.01012-12. Epub 2012 May 2.
3
Rapid and effective diagnosis of tuberculosis and rifampicin resistance with Xpert MTB/RIF assay: a meta-analysis.Xpert MTB/RIF 检测技术快速诊断结核分枝杆菌及利福平耐药性：Meta 分析。
J Infect. 2012 Jun;64(6):580-8. doi: 10.1016/j.jinf.2012.02.012. Epub 2012 Feb 27.
4
Cepheid GeneXpert MTB/RIF assay for Mycobacterium tuberculosis detection and rifampin resistance identification in patients with substantial clinical indications of tuberculosis and smear-negative microscopy results.Cepheid GeneXpert MTB/RIF assay 用于检测结核分枝杆菌和利福平耐药性鉴定，患者有明显的结核病临床指征和痰涂片阴性的显微镜检查结果。
J Clin Microbiol. 2011 Aug;49(8):3068-70. doi: 10.1128/JCM.00718-11. Epub 2011 Jun 15.
5
Tuberculosis of spine.脊柱结核
J Craniovertebr Junction Spine. 2010 Jul;1(2):74-85. doi: 10.4103/0974-8237.77671.
6
Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens.比较 Xpert MTB/RIF 检测与 IS6110-TaqMan 实时 PCR 检测在呼吸道和非呼吸道标本中直接检测结核分枝杆菌的效果。
J Clin Microbiol. 2011 May;49(5):1772-6. doi: 10.1128/JCM.02157-10. Epub 2011 Mar 16.
7
Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system.应用自动化 GeneXpert MTB/RIF 系统快速检测肺外结核。
J Clin Microbiol. 2011 Apr;49(4):1202-5. doi: 10.1128/JCM.02268-10. Epub 2011 Jan 26.
8
Rapid molecular detection of tuberculosis and rifampin resistance.快速分子检测结核分枝杆菌及利福平耐药性。
N Engl J Med. 2010 Sep 9;363(11):1005-15. doi: 10.1056/NEJMoa0907847. Epub 2010 Sep 1.
9
Evaluation of the analytical performance of the Xpert MTB/RIF assay.评估 Xpert MTB/RIF assay 的分析性能。
J Clin Microbiol. 2010 Jul;48(7):2495-501. doi: 10.1128/JCM.00128-10. Epub 2010 May 26.
10
Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology.采用按需、近患者技术快速检测结核分枝杆菌和利福平耐药性。
J Clin Microbiol. 2010 Jan;48(1):229-37. doi: 10.1128/JCM.01463-09. Epub 2009 Oct 28.
Zotero 插件