Prevalence of Thermotolerant spp. in Chicken Meat in Croatia and Multilocus Sequence Typing of a Small Subset of and Isolates.

In order to detect thermotolerant spp., 241 samples of fresh chicken meat, at retail in Croatia, were analysed according to a standard method, followed by biochemical test and molecular polymerase chain reaction/restriction enzyme analysis for exact species determination. spp. prevalence was 73.86%. and were isolated from 53.53 and 15.35% of the samples, respectively. In 4.98% of isolates thermotolerant spp. were not determined. The multilocus sequence typing method was used to evaluate genetic diversity of eight and four isolates. To our knowledge, these results of genotyping provided the first data on the presence of sequence types (STs) and clonal complexes (CCs) of and . isolates in Croatia. By applying the multilocus sequence typing, a new allele of gene locus was discovered and marked 508. The . ST 6182 and . ST 6183 genotypes were described for the first time, and all other identified genotypes were clustered in the previously described sequence types and clonal complexes. These findings provide useful information on the prevalence and epidemiology of and in Croatia.