Zorman T, Heyndrickx M, Uzunović-Kamberović S, Smole Mozina S
University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia.
Int J Food Microbiol. 2006 Jul 1;110(1):24-33. doi: 10.1016/j.ijfoodmicro.2006.03.001. Epub 2006 May 19.
Thermotolerant Campylobacter jejuni and C. coli are one of the major causes of bacterial foodborne enteric infection. Consuming and/or handling poultry meat is the most consistent risk factor, linked to the high prevalence of campylobacters in retail poultry meat. The aim of the present study was to ascertain the genetic diversity and/or possible specificity of thermotolerant Campylobacter isolates according to species (C. coli, C. jejuni), isolation source (retail chicken meat and human clinical samples) and geographic origin (Goriska in Slovenia and Zenica-Doboj Canton in Bosnia and Herzegovina (BIH)). With the pulsed-field gel electrophoresis (PFGE) after SmaI macrorestriction we distinguished 80 PFGE types among 118 strains and CfoI restriction fragment length polymorphism of the amplified flagellin gene (fla-RFLP) gave 12 fla-RFLP types. Beside the higher discriminatory power and strain typeability, PFGE discriminated the C. jejuni and C. coli groups of isolates. A high proportion of C. coli strains was isolated, especially from poultry samples. Identical or very similar PFGE types among the isolates from animal, food and human samples indicate the transmission of C. jejuni and C. coli from the chickens on the farm to the retail chicken meat, as well as possible cross-contamination of retail meat and transmission to humans. However, the identity of the isolates from non-related samples but with identical PFGE and fla-RFLP types should be confirmed with additional typing. Reliable tracing of the source of Campylobacter strains by molecular typing of the chicken meat isolates is therefore very difficult. The reasons include contamination of meat samples with multiple strains, possible cross-contamination and extreme heterogeneity of the isolates (mainly for C. jejuni) on one side and a limited power of the genotyping methods used to distinguish non-related strains on the other side (mainly for C. coli).
耐热空肠弯曲菌和大肠弯曲菌是细菌性食源性肠道感染的主要病因之一。食用和/或处理禽肉是最一致的风险因素，这与零售禽肉中空肠弯曲菌的高流行率有关。本研究的目的是根据菌种（大肠弯曲菌、空肠弯曲菌）、分离源（零售鸡肉和人类临床样本）以及地理来源（斯洛文尼亚的戈里什卡和波斯尼亚和黑塞哥维那（波黑）的泽尼察-多博伊县）确定耐热空肠弯曲菌分离株的遗传多样性和/或可能的特异性。通过SmaI大片段酶切后的脉冲场凝胶电泳（PFGE），我们在118株菌株中区分出80种PFGE型，并且扩增鞭毛蛋白基因的CfoI限制性片段长度多态性（fla-RFLP）产生了12种fla-RFLP型。除了具有更高的鉴别力和菌株分型能力外，PFGE还区分了空肠弯曲菌和大肠弯曲菌分离株组。分离出了高比例的大肠弯曲菌菌株，尤其是从家禽样本中分离出的。动物、食品和人类样本分离株中相同或非常相似的PFGE型表明空肠弯曲菌和大肠弯曲菌从农场鸡传播到零售鸡肉，以及零售肉可能的交叉污染和传播给人类。然而，来自不相关样本但具有相同PFGE和fla-RFLP型的分离株的一致性应通过额外的分型来确认。因此，通过对鸡肉分离株进行分子分型来可靠追踪弯曲菌菌株的来源非常困难。原因一方面包括肉样本被多种菌株污染、可能的交叉污染以及分离株（主要是空肠弯曲菌）的极端异质性，另一方面包括用于区分不相关菌株的基因分型方法的鉴别力有限（主要是大肠弯曲菌）。
