Ayyappa K A, Shatwan I, Bodhini D, Bramwell L R, Ramya K, Sudha V, Anjana R M, Lovegrove J A, Mohan V, Radha V, Vimaleswaran K S
Department of Molecular Genetics, Madras Diabetes Research Foundation, Kallam Anji Reddy Centre, Plot no. 20, Golden Jubilee Biotech Park for Women Society, SIPCOT-IT PARK, Siruseri, Chennai 603 103 India.
Present Address: Department of Biotechnology, School of Chemical & Biotechnology, SASTRA University, Tanjore, India.
Nutr Metab (Lond). 2017 Jan 19;14:8. doi: 10.1186/s12986-016-0155-1. eCollection 2017.
BACKGROUND: Single nucleotide polymorphisms (SNPs) in lipoprotein lipase gene () have been shown to influence metabolism related to lipid phenotypes. Dietary factors have been shown to modify the association between SNPs and lipids; however, to date, there are no studies in South Asians. Hence, we tested for the association of four common SNPs with plasma lipids and examined the interactions between the SNPs and dietary factors on lipids in 1,845 Asian Indians. METHODS: The analysis was performed in 788 Type 2 diabetes cases and 1,057 controls randomly chosen from the cross-sectional Chennai Urban Rural Epidemiological Study. Serum triacylglycerol (TAG), serum total cholesterol, and high-density lipoprotein cholesterol (HDL-C) were measured using a Hitachi-912 autoanalyzer (Roche Diagnostics GmbH, Mannheim, Germany). Dietary intake was assessed using a semi-quantitative food frequency questionnaire. The SNPs (rs1121923, rs328, rs4922115 and rs285) were genotyped by polymerase chain reaction followed by restriction enzyme digestion and 20% of samples were sequenced to validate the genotypes obtained. Statistical Package for Social Sciences for Windows version 22.0 (SPSS, Chicago, IL) was used for statistical analysis. RESULTS: After correction for multiple testing and adjusting for potential confounders, SNPs rs328 and rs285 showed association with HDL-C ( = 0.0004) and serum TAG ( = 1×10), respectively. The interaction between SNP rs1121923 and fat intake (energy %) on HDL-C ( = 0.003) was also significant, where, among those who consumed a high fat diet (28.4 ± 2.5%), the T allele carriers (TT + XT) had significantly higher HDL-C concentrations ( = 0.0002) and 30% reduced risk of low HDL-C levels compared to the CC homozygotes. None of the interactions on other lipid traits were statistically significant. CONCLUSION: Our findings suggest that individuals carrying T allele of the SNP rs1121923 have increased HDL-C levels when consuming a high fat diet compared to CC homozygotes. Our finding warrants confirmation in prospective studies and randomized controlled trials.
背景：脂蛋白脂肪酶基因（）中的单核苷酸多态性（SNPs）已被证明会影响与脂质表型相关的代谢。饮食因素已被证明会改变SNPs与脂质之间的关联；然而，迄今为止，南亚地区尚无相关研究。因此，我们在1845名亚洲印度人中测试了4种常见的SNPs与血浆脂质的关联，并研究了这些SNPs与饮食因素在脂质方面的相互作用。 方法：分析在从钦奈城乡流行病学横断面研究中随机选取的788例2型糖尿病患者和1057名对照中进行。使用日立912自动分析仪（德国曼海姆罗氏诊断有限公司）测量血清三酰甘油（TAG）、血清总胆固醇和高密度脂蛋白胆固醇（HDL-C）。使用半定量食物频率问卷评估饮食摄入量。通过聚合酶链反应，随后进行限制性内切酶消化对SNPs（rs1121923、rs328、rs4922115和rs285）进行基因分型，并对20%的样本进行测序以验证所获得的基因型。使用Windows版社会科学统计软件包22.0（SPSS，伊利诺伊州芝加哥）进行统计分析。 结果：在进行多重检验校正并调整潜在混杂因素后，SNPs rs328和rs285分别与HDL-C（=0.0004）和血清TAG（=1×10）显示出关联。SNP rs1121923与脂肪摄入量（能量百分比）对HDL-C的相互作用（=0.003）也具有显著性，其中，在食用高脂肪饮食（28.4±2.5%）的人群中，T等位基因携带者（TT+XT）的HDL-C浓度显著更高（=0.0002），与CC纯合子相比，HDL-C水平低的风险降低了30%。其他脂质性状的相互作用均无统计学显著性。 结论：我们的研究结果表明，与CC纯合子相比，携带SNP rs1121923的T等位基因的个体在食用高脂肪饮食时HDL-C水平升高。我们的发现有待前瞻性研究和随机对照试验予以证实。
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