Gambatesa V
Medicina (Firenze). 1989 Apr-Jun;9(2):184-7.
Human T-T hybridomas were obtained by fusing T lymphoblasts of the azaguanine-resistant CEM-6 cell line and PHA-activated normal T lymphocytes. After 4 weeks of culture with selective media, proliferating hybrid cells were cloned by limiting dilution. Several clones were obtained and one of them (F2) was shown to secrete a lymphokine with inhibitory activity on the proliferation of an EBV-transformed B cell line, of normal B lymphocytes, as well as of mitogen activated normal T lymphocytes. The proliferation of three other cell lines (MLA-144, CEM-6 and K-562) was not affected. The inhibition of cell proliferation was not due to a cytotoxic effect. The soluble factor from F2 hybridoma was very sensitive to heat-treatment, was not inactivated by target cell adsorption and, finally, it was effective at very low concentrations (70% inhibition at 1:1,000 dilution, v/v). The suppressor activity in the supernatant from F2 hybridoma did not appear to be related to other known lymphokines.
人T - T杂交瘤是通过将抗氮杂鸟嘌呤的CEM - 6细胞系的T淋巴母细胞与PHA激活的正常T淋巴细胞融合而获得的。在用选择性培养基培养4周后，通过有限稀释法对增殖的杂交细胞进行克隆。获得了几个克隆，其中一个克隆（F2）被证明能分泌一种对EB病毒转化的B细胞系、正常B淋巴细胞以及丝裂原激活的正常T淋巴细胞的增殖具有抑制活性的淋巴因子。其他三种细胞系（MLA - 144、CEM - 6和K - 562）的增殖不受影响。细胞增殖的抑制并非由于细胞毒性作用。来自F2杂交瘤的可溶性因子对热处理非常敏感，不会因靶细胞吸附而失活，最后，它在极低浓度下也有效（1:1000稀释，v/v时抑制率为70%）。F2杂交瘤上清液中的抑制活性似乎与其他已知淋巴因子无关。
