用于过继性转移的免疫细胞分离
Isolation of Immune Cells for Adoptive Transfer.
作者信息
Barhoumi Tlili, Paradis Pierre, Mann Koren K, Schiffrin Ernesto L
机构信息
Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC, Canada.
Department of Oncology, Jewish General Hospital, McGill University, Montreal, QC, Canada.
出版信息
Methods Mol Biol. 2017;1527:321-344. doi: 10.1007/978-1-4939-6625-7_25.
Adoptive transfer of T lymphocytes is a useful technique to characterize the role of the immune system in hypertension and vascular disease. Here we describe as an example the isolation of splenic T regulatory cells from donor mice processed to obtain a single cell suspension, followed by negative and positive selection to obtain CD4 T cells and CD4CD25 Treg cells, respectively. Treg cells can be subsequently transferred to recipient animals.
T淋巴细胞的过继转移是一种用于表征免疫系统在高血压和血管疾病中作用的有用技术。在此，我们以从供体小鼠中分离脾调节性T细胞为例进行描述，首先将供体小鼠处理以获得单细胞悬液，随后进行阴性和阳性分选，分别获得CD4 T细胞和CD4CD25调节性T细胞。调节性T细胞随后可转移至受体动物体内。
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