小血管和大血管血管平滑肌细胞的分离与培养
Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels.
作者信息
Montezano Augusto C, Lopes Rheure A M, Neves Karla B, Rios Francisco, Touyz Rhian M
机构信息
Institute of Cardiovascular and Medical Sciences BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
Kidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.
出版信息
Methods Mol Biol. 2017;1527:349-354. doi: 10.1007/978-1-4939-6625-7_27.
Primary culture of vascular smooth muscle cells is an important in vitro model for the dissection of molecular mechanisms related to a specific physiological or pathological response at the cellular level. Cultured cells also provide an excellent model to study cell biology. This chapter describes a user-friendly and practical protocol for isolation of vascular smooth muscle cells from small and large vessels by enzymatic dissociation, which can be applied to vessels from different species, including rodents and humans.
血管平滑肌细胞的原代培养是在细胞水平剖析与特定生理或病理反应相关分子机制的重要体外模型。培养的细胞也为研究细胞生物学提供了一个优秀的模型。本章描述了一种通过酶解从大小血管中分离血管平滑肌细胞的简便实用方法，该方法可应用于包括啮齿动物和人类在内的不同物种的血管。
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