Vu Hai M T, Warembourg M, Milgrom E
Ann N Y Acad Sci. 1977 Mar 11;286:199-209. doi: 10.1111/j.1749-6632.1977.tb29417.x.
Uterine progesterone receptors are under dual hormonal control. Estrogen increases the concentration of receptor through a mechanism that depends on synthesis of both RNA and protein. Progesterone decreases the concentration of its own receptor, probably by enhancing its inactivation rate. This regulation explains receptor variations during the estrous cycle. In both guinea pig and rat uteri, cytosol receptor concentration is maximal at the preovulatory period and decreases after ovulation. Nuclear receptor was measured in the rat. Its concentration is also maximal at proestrus, but the higher nuclear to cytosol receptor ratio was observed at metestrus. There is a good correlation (r = 0.78) between nuclear receptor concentration, on one hand, and the product of cytosol receptor concentration times the plasma progesterone concentration, on the other hand. Autoradiographic studies show that receptor variations during the estrous cycle occur simultaneously in all cell types of uterine horn, cervix, and vagina, which suggests that similar mechanisms control receptor concentration in all of these cells. Progesterone receptor was also measured during pregnancy in rat uterus. Cytosol receptor concentration is low at the beginning of pregnancy (approximately 6000 binding sites per cell), declines slightly on Day 5 (approximately 4000 binding sites per cell), and then increases progressively during the remainder of pregnancy to attain its highest value on Day 22 (26,000 binding sites per cell). Nuclear receptor concentration is very low on Day 3 (1200 binding sites per cell), increases slightly on Day 5 (1900 binding sites per cell), decreases on Day 6, and then increases again to attain a plateau between Days 9 and 15 (approximately 2600 binding sites per cell). Thereafter, its concentration begins to decrease rapidly. On Day 22, the mean concentration is very low (700 binding sites per cell); in some animals (probably on the verge of parturition), no nuclear receptor can be detected.
子宫孕激素受体受双重激素控制。雌激素通过一种依赖于RNA和蛋白质合成的机制增加受体浓度。孕激素可能通过提高其失活速率来降低自身受体的浓度。这种调节解释了发情周期中受体的变化。在豚鼠和大鼠子宫中，胞质溶胶受体浓度在排卵前期最高，排卵后降低。对大鼠的核受体进行了测定。其浓度在动情前期也最高，但在动情后期观察到核受体与胞质溶胶受体的比例更高。一方面，核受体浓度与另一方面胞质溶胶受体浓度乘以血浆孕激素浓度的乘积之间存在良好的相关性（r = 0.78）。放射自显影研究表明，发情周期中受体的变化在子宫角、子宫颈和阴道的所有细胞类型中同时发生，这表明类似的机制控制着所有这些细胞中的受体浓度。还对大鼠孕期子宫中的孕激素受体进行了测定。妊娠开始时胞质溶胶受体浓度较低（约每细胞6000个结合位点），在第5天略有下降（约每细胞4000个结合位点），然后在妊娠剩余时间逐渐增加，在第22天达到最高值（每细胞26000个结合位点）。第3天核受体浓度非常低（每细胞1200个结合位点），第5天略有增加（每细胞1900个结合位点），第6天下降，然后再次增加，在第9天至第15天达到平台期（约每细胞2600个结合位点）。此后，其浓度开始迅速下降。在第22天，平均浓度非常低（每细胞700个结合位点）；在一些动物中（可能处于分娩边缘），无法检测到核受体。
