Yuan Hongbo, Xing Chengfen, Fan Yibing, Chai Ran, Niu Ruimin, Zhan Yong, Peng Fei, Qi Junjie
School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300401, P. R. China.
Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, Hebei University of Technology, Tianjin, 300401, P. R. China.
Macromol Rapid Commun. 2017 Mar;38(5). doi: 10.1002/marc.201600726. Epub 2017 Jan 24.
The CO -responsive and biocatalytic assembly based on conjugated polymers has been demonstrated by combining the signal amplification property of the polythiophene derivative (PTP) and the catalytic actions of carbonic anhydrase (CA). CO is applied as a new trigger mode to construct the smart assembly by controlling the electrostatic and hydrophobic interactions between the PTP molecules in aqueous solution, leading to the visible fluorescence changes. Importantly, the assembly transformation of PTP can be specifically and highly accelerated by CA based on the efficient catalytic activity of CA for the inter-conversion between CO and HCO , mimicking the CO -associated biological processes that occurred naturally in living organisms. Moreover, the PTP-based assembly can be applied for biomimetic CO sequestration with fluorescence monitoring in the presence of CA and calcium.
通过结合聚噻吩衍生物（PTP）的信号放大特性和碳酸酐酶（CA）的催化作用，已证明了基于共轭聚合物的CO响应性和生物催化组装。CO作为一种新的触发模式，通过控制水溶液中PTP分子之间的静电和疏水相互作用来构建智能组装体，从而导致可见荧光变化。重要的是，基于CA对CO和HCO之间相互转化的高效催化活性，PTP的组装体转变可被CA特异性且高度加速，模拟了生物体中自然发生的与CO相关的生物过程。此外，基于PTP的组装体可用于在CA和钙存在下进行荧光监测的仿生CO封存。
