Ziegler Thomas R, Judd Suzanne E, Ruff Joshua H, McComsey Grace A, Eckard Allison Ross
1 Department of Medicine, Emory University School of Medicine , Atlanta, Georgia .
2 Department of Biostatistics, University of Alabama at Birmingham , Birmingham, Alabama.
AIDS Res Hum Retroviruses. 2017 Jul;33(7):681-689. doi: 10.1089/AID.2015.0369. Epub 2017 Feb 27.
Amino acids play critical roles in metabolism, cell function, body composition and immunity, but little data on plasma amino acid concentrations in HIV are available. We evaluated plasma amino acid concentrations and associations with CD4 counts and inflammatory biomarkers in HIV-infected youth. HIV-infected subjects with a high (≥500 cells/mm) and low (<500 cells/mm) current CD4 T cell counts were compared to one another and to a matched healthy control group. Plasma concentrations of 19 amino acids were determined with an amino acid analyzer. Plasma levels of interleukin-6, tumor necrosis factor receptor-I, and soluble vascular cellular adhesion molecule-I were also measured. Seventy-nine HIV-infected subjects (40 and 39 with high and low CD4 T cell counts, respectively) and 40 controls were included. There were no differences in amino acid concentrations between HIV-infected subjects with high or low CD4 T cell counts. When combined, the HIV-infected group exhibited significantly lower median plasma concentrations compared to controls for total, essential, branched-chain and sulfur amino acids, as well as for 12 individual amino acids. Glutamate was the only amino acid that was higher in the HIV-infected group. There were no significant correlations between amino acid endpoints and inflammatory biomarkers for either HIV-infected group or controls. Plasma amino acid concentrations were lower in HIV-infected youth compared to healthy controls, regardless of immune status, while glutamate concentrations were elevated. These findings can inform future interventional studies designed to improve metabolic and clinical parameters influenced by amino acid nutriture.
氨基酸在新陈代谢、细胞功能、身体组成和免疫中发挥着关键作用，但关于艾滋病毒感染者血浆氨基酸浓度的数据却很少。我们评估了艾滋病毒感染青年的血浆氨基酸浓度及其与CD4细胞计数和炎症生物标志物的关联。将当前CD4 T细胞计数高（≥500个细胞/mm³）和低（<500个细胞/mm³）的艾滋病毒感染受试者相互比较，并与匹配的健康对照组进行比较。使用氨基酸分析仪测定19种氨基酸的血浆浓度。还测量了白细胞介素-6、肿瘤坏死因子受体-I和可溶性血管细胞粘附分子-I的血浆水平。纳入了79名艾滋病毒感染受试者（分别有40名和39名CD4 T细胞计数高和低的受试者）和40名对照组。CD4 T细胞计数高或低的艾滋病毒感染受试者之间的氨基酸浓度没有差异。合并后，艾滋病毒感染组与对照组相比，总氨基酸、必需氨基酸、支链氨基酸和含硫氨基酸以及12种单个氨基酸的血浆中位数浓度显著较低。谷氨酸是艾滋病毒感染组中唯一较高的氨基酸。艾滋病毒感染组或对照组的氨基酸终点与炎症生物标志物之间均无显著相关性。无论免疫状态如何，艾滋病毒感染青年的血浆氨基酸浓度均低于健康对照组，而谷氨酸浓度升高。这些发现可为未来旨在改善受氨基酸营养影响的代谢和临床参数的干预研究提供参考。
