Baffa A F, Camara D C P, Santos-Mallet J R, DA Silva E R, Costa J, Freitas S P C
Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil.
Laboratório de Insetos Aquáticos, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
Med Vet Entomol. 2017 Jun;31(2):192-199. doi: 10.1111/mve.12226. Epub 2017 Jan 24.
Morphological and structural features of the sperm of the Triatoma brasiliensis Neiva, 1911 (Hemiptera: Reduviidae) species complex were examined in this first study investigating the sperm of Heteroptera and the genus Triatoma. Males were dissected and their seminal vesicles removed. For measurement, seminal vesicles were squashed on glass slides, spread, fixed and observed under a photomicroscope. The images were analysed and measures of sperm were made. Data were analysed using one-way analysis of variance and Tukey's test to detect differentiation among taxa. Furthermore, seminal vesicles were prepared for studies of transmission electron microscopy. All taxa studied showed polymorphic (short and long) sperm. The sperm of Triatoma brasiliensis macromelasoma was significantly longer (in total length) than that of the other four members of the complex, which supports the hypothesis of hybrid speciation of this member of the complex as an increase in the size of typical hybrids under heterosis was previously shown. The sperm cells of the five taxa have similar ultrastructural morphology. The ultrastructural features observed confirm the hypothesis, raised by previous studies, that they are synapomorphic to the suborder Heteroptera.
在这项关于异翅目昆虫及锥蝽属的精子的首次研究中，对1911年的巴西锥蝽（Triatoma brasiliensis Neiva）（半翅目：猎蝽科）物种复合体的精子的形态学和结构特征进行了研究。解剖雄性个体并取出其精囊。为了进行测量，将精囊压在载玻片上，展开、固定并在光学显微镜下观察。对图像进行分析并测量精子。使用单因素方差分析和图基检验分析数据以检测不同分类单元之间的差异。此外，制备精囊用于透射电子显微镜研究。所有研究的分类单元都显示出多态性（短和长）精子。巴西锥蝽大黑斑亚种（Triatoma brasiliensis macromelasoma）的精子（总长度）明显长于该复合体的其他四个成员，这支持了该复合体这一成员杂交物种形成的假说，因为先前已表明杂种优势下典型杂种的大小会增加。五个分类单元的精子细胞具有相似的超微结构形态。观察到的超微结构特征证实了先前研究提出的假说，即它们是异翅亚目的共同衍征。
