GPER启动子甲基化调控乳腺癌患者的GPER表达。
GPER Promoter Methylation Controls GPER Expression in Breast Cancer Patients.
作者信息
Weissenborn Christine, Ignatov Tanja, Nass Norbert, Kalinski Thomas, Dan Costa Serban, Zenclussen Ana Claudia, Ignatov Atanas
机构信息
a Department of Obstetrics and Gynecology , Otto-von-Guericke University , Magdeburg , Germany.
b Department of Experimental Obstetrics and Gynaecology , Otto-von-Guericke University , Magdeburg , Germany.
出版信息
Cancer Invest. 2017 Feb 7;35(2):100-107. doi: 10.1080/07357907.2016.1271886. Epub 2017 Jan 24.
Recently, we found that G-protein-coupled estrogen receptor (GPER) protein expression decreased during breast carcinogenesis, and that GPER promoter is methylated. Here we analyzed GPER promoter methylation in 260 primary breast cancer specimens by methylation-specific polymerized chain reaction. The results demonstrated that GPER protein down-regulation significantly correlated with GPER promoter hypermethylation (p < .001). Comparison of 108 tumors and matched normal breast tissues indicated a significant GPER down-regulation in cancer tissues correlating with GPER promoter hypermethylation (p < .001). The latter was an unfavorable factor for overall survival of patients with triple-negative breast cancer (p = .025). Thus GPER promoter hypermethylation might be used as a prognostic factor.
最近，我们发现G蛋白偶联雌激素受体（GPER）蛋白表达在乳腺癌发生过程中降低，并且GPER启动子发生甲基化。在此，我们通过甲基化特异性聚合酶链反应分析了260例原发性乳腺癌标本中GPER启动子的甲基化情况。结果表明，GPER蛋白下调与GPER启动子高甲基化显著相关（p <.001）。对108例肿瘤组织及其配对的正常乳腺组织进行比较，结果显示癌组织中GPER显著下调，且与GPER启动子高甲基化相关（p <.001）。后者是三阴性乳腺癌患者总生存的不利因素（p =.025）。因此，GPER启动子高甲基化可能用作预后因素。
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