Kylli Petri, Hankemeier Thomas, Kostiainen Risto
Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, FI-00014, Finland.
Division of Analytical Biosciences, Leiden Academic Centre for Drug Research, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands; Netherlands Metabolomics Centre, Leiden University, P.O. Box 9502, 22300 RA, Leiden, The Netherlands.
J Chromatogr A. 2017 Mar 3;1487:147-152. doi: 10.1016/j.chroma.2017.01.039. Epub 2017 Jan 18.
Oxysterols are oxygenated cholesterols that are important in many cell functions and they may also be indicative of certain diseases. The purpose of this work was to study the feasibility of ultra-performance liquid chromatography-ion mobility-time-of-flight mass spectrometry (UPLC-IM-TOFMS) using traveling wave cell in analyzing oxysterols and especially their isomers in biological samples. Oxysterols were analyzed as their p-toluenesulfonyl isocyanate derivatives, which improved the separation of isomeric oxysterols by ion mobility and ionization efficiency in the electrospray ionization step. The UPLC-IM-TOFMS method was shown to be fast and to provide good quantitative performance. The feasibility of the method was demonstrated in the analyses of oxysterols in fibroblast cell samples.
氧化甾醇是氧化胆固醇，在许多细胞功能中起重要作用，它们也可能是某些疾病的指示物。这项工作的目的是研究使用行波池的超高效液相色谱 - 离子淌度 - 飞行时间质谱法（UPLC - IM - TOFMS）分析生物样品中氧化甾醇尤其是其异构体的可行性。氧化甾醇作为对甲苯磺酰异氰酸酯衍生物进行分析，这在电喷雾电离步骤中通过离子淌度和电离效率改善了异构氧化甾醇的分离。结果表明，UPLC - IM - TOFMS方法快速且具有良好的定量性能。该方法的可行性在成纤维细胞样品中氧化甾醇的分析中得到了证明。
