Iusibov V M, Pogosian G P, Andrianov V M, Piruzian E S
Mol Gen Mikrobiol Virusol. 1989 Jul(7):11-3.
The gene transfer into plants using the genetic engineering methods gives us the possibility to obtain transgeneric plants having acquired the new traits. Some bacterial genes can be used for this purpose. Obtaining of a transgeneric plant harbouring the cytokinin synthesis gene ipt (gene 4) from the T-DNA of Agrobacterium tumefaciens Ti-plasmid seems to be useful. The expression of tumor agrobacterial ipt gene in transformed plant cells interferes with the normal growth and regulation of the whole plant. The successful transfer of the cloned ipt gene from the recombinant plasmid pGV0319 into the tobacco plant using Agrobacterium vectors and succeeding regeneration of phenotypically normal transgenic plants are reported in the present paper.
利用基因工程方法将基因导入植物，使我们有机会获得具有新性状的转基因植物。一些细菌基因可用于此目的。从根癌土壤杆菌Ti质粒的T-DNA中获得携带细胞分裂素合成基因ipt（基因4）的转基因植物似乎很有用。肿瘤农杆菌ipt基因在转化植物细胞中的表达会干扰整个植物的正常生长和调控。本文报道了利用农杆菌载体将克隆的ipt基因从重组质粒pGV0319成功导入烟草植株，并成功再生出表型正常的转基因植株。
