Xie Shiwei, Tian Lixia, Niu Jin, Liang Guiying, Liu Yongjian
Nutrition Laboratory, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Fish Physiol Biochem. 2017 Aug;43(4):1011-1020. doi: 10.1007/s10695-017-0348-1. Epub 2017 Jan 25.
An 8-week feeding trial was conducted to evaluate the effect of N-acetyl cysteine (NAC) and glycine supplementation on growth performance, glutathione (GSH) synthesis, and antioxidative ability of grass carp, Ctenopharyngodon idella. Four practical diets were formulated: control, control + 0.2% NAC, control + 0.5% glycine, and control + 0.2% NAC + 0.5% glycine. Each diet was randomly assigned to quadruplicate groups of 30 fish (approximately 8.8 g). Weight gain and specific growth rate were significantly increased with the supplementation of NAC. Supplementation of NAC plus glycine significantly increased the feed efficiency. Glutathione peroxidase (GPx) and γ-glutamine cysteine synthase (γ-GCS) in plasma were significantly increased with the supplementation of NAC plus glycine. GSH in plasma increased and malondialdehyde (MDA) decreased in fish fed diets supplemented with NAC. Respiratory burst, superoxide dismutase (SOD), and catalase (CAT) activity were not affected by NAC or glycine. These results clearly indicated that NAC improved the growth performance and restored GSH of grass carp, supplemented NAC together with glycine enhanced GSH synthesis, and improved the antioxidative ability of grass carp.
进行了一项为期8周的饲养试验，以评估补充N-乙酰半胱氨酸（NAC）和甘氨酸对草鱼（Ctenopharyngodon idella）生长性能、谷胱甘肽（GSH）合成及抗氧化能力的影响。配制了四种实用饲料：对照组、对照组 + 0.2% NAC、对照组 + 0.5%甘氨酸以及对照组 + 0.2% NAC + 0.5%甘氨酸。每种饲料随机分配到四组，每组30尾鱼（约8.8克）。补充NAC后，增重和特定生长率显著提高。补充NAC加甘氨酸显著提高了饲料效率。补充NAC加甘氨酸后，血浆中的谷胱甘肽过氧化物酶（GPx）和γ-谷氨酰半胱氨酸合成酶（γ-GCS）显著增加。在补充NAC的饲料喂养的鱼中，血浆中GSH增加，丙二醛（MDA）减少。呼吸爆发、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性不受NAC或甘氨酸的影响。这些结果清楚地表明，NAC改善了草鱼的生长性能并恢复了其GSH水平，NAC与甘氨酸一起补充可增强GSH合成，并提高草鱼的抗氧化能力。
