Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice.

Identification of related mutants contributed a lot to our understanding of the molecular mechanisms underlying brassinosteroid (BR) signaling. Establishment of methods to test for the hormone sensitivity is essential for the characterization of the mutants associated with BR responses. Here, we describe detailed procedures for the physiological analysis of BR responses that are regularly applied to test the BR sensitivity of mutants by evaluating the growth of different tissues of rice (Oryza sativa) seedlings.