水稻中油菜素内酯反应与敏感性的生理分析
Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice.
作者信息
Tong Hongning, Chu Chengcai
机构信息
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, 100101, China.
出版信息
Methods Mol Biol. 2017;1564:23-29. doi: 10.1007/978-1-4939-6813-8_3.
Identification of related mutants contributed a lot to our understanding of the molecular mechanisms underlying brassinosteroid (BR) signaling. Establishment of methods to test for the hormone sensitivity is essential for the characterization of the mutants associated with BR responses. Here, we describe detailed procedures for the physiological analysis of BR responses that are regularly applied to test the BR sensitivity of mutants by evaluating the growth of different tissues of rice (Oryza sativa) seedlings.
相关突变体的鉴定对我们理解油菜素内酯（BR）信号传导的分子机制有很大帮助。建立检测激素敏感性的方法对于鉴定与BR反应相关的突变体至关重要。在这里，我们描述了BR反应生理分析的详细程序，这些程序通常用于通过评估水稻（Oryza sativa）幼苗不同组织的生长来测试突变体的BR敏感性。
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