通过染色质免疫沉淀结合高通量测序（ChIP-seq）和RNA测序鉴定油菜素内酯靶基因。
Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing.
作者信息
Nolan Trevor, Liu Sanzhen, Guo Hongqing, Li Lei, Schnable Patrick, Yin Yanhai
机构信息
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, 50011-3650, USA.
Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506-5502, USA.
出版信息
Methods Mol Biol. 2017;1564:63-79. doi: 10.1007/978-1-4939-6813-8_7.
Brassinosteroids (BRs) play important roles in many growth and developmental processes. BRs signal to regulate BR-INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulate several hundreds of transcription factors (termed BES1/BZR1-targeted TFs or BTFs) and thousands of genes to mediate various BR responses. Chromatin Immunoprecipitation followed by high-throughput sequencing (ChIP-seq) with BES1/BZR1 and BTFs is an important approach to identify BR target genes. In combination with RNA-sequencing experiments, these genomic methods have become powerful tools to detect BR target genes and reveal transcriptional networks underlying BR-regulated processes.
油菜素甾醇（BRs）在许多生长和发育过程中发挥着重要作用。BRs信号传导以调节BR不敏感1-乙基甲磺酸酯抑制因子1（BES1）和油菜素唑抗性1（BZR1）转录因子（TFs），反过来，这些转录因子又调节数百种转录因子（称为BES1/BZR1靶向TFs或BTFs）和数千个基因，以介导各种BR反应。用BES1/BZR1和BTFs进行染色质免疫沉淀后高通量测序（ChIP-seq）是鉴定BR靶基因的重要方法。结合RNA测序实验，这些基因组方法已成为检测BR靶基因和揭示BR调控过程潜在转录网络的强大工具。
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