School of Environmental Science and Engineering, Shandong University, Jinan 250100, China.
School of Environmental Science and Engineering, Shandong University, Jinan 250100, China; Shandong Provincial Engineering Center on Environmental Science and Technology, Jinan, 250061, China.
Sci Total Environ. 2017 Apr 1;583:496-504. doi: 10.1016/j.scitotenv.2017.01.104. Epub 2017 Jan 23.
This work was aimed toward studying the new application of chitosan quaternary ammonium salt (HTCC), a water-soluble chitosan derivative, on removal of Microcystis aeruginosa (M. aeruginosa) cells during HTCC coagulation and floc storage. Results showed that all cells were removed without damage under optimum coagulation conditions: HTCC dosage 1.5mg/L, rapid mixing for 0.5min at 5.04g and slow mixing for 30min at 0.20g. The high removal efficiency was due to the large size and compact structure of flocs formed by HTCC, which readily settled. During floc storage, HTCC could induce production of reactive oxygen species (ROS), which would accelerate M. aeruginosa cell lysis. But the flocs, into which the cells aggregated, could protect cells from cellular oxidative damage caused by ROS, thus keeping the cells intact for a longer time.
本研究旨在探讨壳聚糖季铵盐（HTCC）的新应用，这是一种水溶性壳聚糖衍生物，可用于去除 HTCC 混凝和絮体储存过程中的铜绿微囊藻（M. aeruginosa）细胞。结果表明，在最佳混凝条件下，所有细胞均能被去除而不受到损伤：HTCC 剂量为 1.5mg/L，快速搅拌 0.5min，速度为 5.04g，慢速搅拌 30min，速度为 0.20g。高去除效率归因于 HTCC 形成的絮体尺寸大且结构紧凑，易于沉降。在絮体储存过程中，HTCC 可诱导活性氧（ROS）的产生，从而加速铜绿微囊藻细胞的裂解。但是，细胞聚集的絮体可以保护细胞免受 ROS 引起的细胞氧化损伤，从而使细胞更长时间保持完整。
