Clifford Alexander M, Bury Nicolas R, Schultz Aaron G, Ede James D, Goss Brendan L, Goss Greg G
Bamfield Marine Sciences Centre, Bamfield, BC, Canada; Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
Bamfield Marine Sciences Centre, Bamfield, BC, Canada; Diabetes & Nutritional Sciences Division, Kings College, London, UK; Faculty of Health and Life Sciences, University of Suffolk, Suffolk, UK.
Gen Comp Endocrinol. 2017 Jun 1;247:107-115. doi: 10.1016/j.ygcen.2017.01.022. Epub 2017 Jan 23.
The goal of this study was to identify whether Pacific hagfish (Eptatretus stoutii) possess glucocorticoid and mineralocorticoid responses and to examine the potential role(s) of four key steroids in these responses. Pacific hagfish were injected with varying amounts of cortisol, corticosterone or 11-deoxycorticosterone (DOC) using coconut oil implants and plasma glucose and gill total-ATPase activity were monitored as indices of glucocorticoid and mineralocorticoid responses. Furthermore, we also monitored plasma glucose and 11-deoxycortisol (11-DOC) levels following exhaustive stress (30 min of agitation) or following repeated infusion with SO. There were no changes in gill total-ATPase following implantation with any steroid, with only very small statistical increases in plasma glucose noted in hagfish implanted with either DOC (at 20 and 200mgkg at 7 and 4days post-injection, respectively) or corticosterone (at 100mgkg at 7days post-injection). Following exhaustive stress, hagfish displayed a large and sustained increase in plasma glucose. Repeated infusion of SO into hagfish caused increases in both plasma glucose levels and SO excretion rate suggesting a regulated glucocorticoid and mineralocorticoid response. However, animals under either condition did not show any significant increases in plasma 11-DOC concentrations. Our results suggest that while there are active glucocorticoid and mineralocorticoid responses in hagfish, 11-DOC does not appear to be involved and the identity and primary function of the steroid in hagfish remains to be elucidated.
本研究的目的是确定太平洋盲鳗（Eptatretus stoutii）是否具有糖皮质激素和盐皮质激素反应，并研究四种关键类固醇在这些反应中的潜在作用。使用椰子油植入物给太平洋盲鳗注射不同剂量的皮质醇、皮质酮或11-脱氧皮质酮（DOC），并监测血浆葡萄糖和鳃总ATP酶活性，作为糖皮质激素和盐皮质激素反应的指标。此外，我们还监测了在进行彻底应激（30分钟搅动）后或反复输注SO后的血浆葡萄糖和11-脱氧皮质醇（11-DOC）水平。植入任何一种类固醇后，鳃总ATP酶均无变化，仅在植入DOC（分别在注射后7天和4天，剂量为20和200mg/kg）或皮质酮（注射后7天，剂量为100mg/kg）的盲鳗中，血浆葡萄糖有非常小的统计学升高。在彻底应激后，盲鳗的血浆葡萄糖出现大幅且持续的升高。向盲鳗反复输注SO导致血浆葡萄糖水平和SO排泄率均升高，表明存在受调节的糖皮质激素和盐皮质激素反应。然而，在这两种情况下，动物的血浆11-DOC浓度均未出现任何显著升高。我们的结果表明，虽然盲鳗存在活跃的糖皮质激素和盐皮质激素反应，但11-DOC似乎未参与其中，盲鳗中该类固醇的身份和主要功能仍有待阐明。
