Huang Yan, Ou Danyun, Chen Shunyang, Chen Bin, Liu Wenhua, Bai Renao, Chen Guangcheng
Marine Biology Institute, Shantou University, Shantou, Guangdong, China; Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian, China.
Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian, China.
Mar Pollut Bull. 2017 Mar 15;116(1-2):434-439. doi: 10.1016/j.marpolbul.2017.01.030. Epub 2017 Jan 23.
The effects of zinc (Zn) on nitrous oxide (NO) fluxes from coastal loam soil and the abundances of soil nitrifier and denitrifier were studied in a tidal microcosm receiving livestock wastewater with different Zn levels. Soil NO emission significantly increased due to discharge of wastewater rich in ammonia (NH-N) while the continuous measurements of gas flux showed a durative reduction in NO flux by high Zn input (40mgL) during the low tide period. Soil inorganic nitrogen concentrations increased at the end of the experiment and even more soil NH-N was measured in the high-Zn-level treatment, indicating an inhibition of ammonia oxidation by Zn input. Quantitative PCR of soil amoA, narG and nirK genes encoding ammonia monooxygenase, nitrate reductase and nitrite reductase, respectively, showed that the microbial abundances involved in these metabolisms were neither affected by wastewater discharge nor Zn contamination.
在一个接收不同锌水平畜禽废水的潮汐微观系统中，研究了锌（Zn）对滨海壤土中氧化亚氮（NO）通量以及土壤硝化菌和反硝化菌丰度的影响。富含氨（NH-N）的废水排放导致土壤NO排放显著增加，而气体通量的连续测量表明，在低潮期，高锌输入（40mg/L）使NO通量持续降低。实验结束时土壤无机氮浓度增加，在高锌水平处理中测得更多的土壤NH-N，表明锌输入抑制了氨氧化。分别对编码氨单加氧酶、硝酸盐还原酶和亚硝酸还原酶的土壤amoA、narG和nirK基因进行定量PCR分析，结果表明参与这些代谢的微生物丰度既不受废水排放的影响，也不受锌污染的影响。
