Poonja Ali, Heirali Alya, Workentine Matthew, Storey Douglas G, Somayaji Ranjani, Rabin Harvey R, Surette Michael G, Parkins Michael D
Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Canada.
Department of Veterinary Sciences, University of Calgary, Calgary, AB, Canada.
J Cyst Fibros. 2017 May;16(3):353-357. doi: 10.1016/j.jcf.2017.01.004. Epub 2017 Jan 24.
Pseudomonas aeruginosa develops profound population heterogeneity in CF airways. How changes in these populations relate to clinical status is unknown. In order to facilitate this understanding, frequent sampling of this community is required. To determine if the collection and storage of sputum at home may pose a viable option, we collected sputum from ten patients. Sputum samples were partitioned in two, with half immediately processed on MacConkey agar and half assessed after freezing for one week in a home-freezer. From each sample, 88 isolates were assessed for antibiotic susceptibility and virulence factor production. Freezing resulted in a 10CFU/ml drop in P. aeruginosa. However, across 1760 isolates, no consistent difference in either antibiotic susceptibility nor virulence factors was observed suggesting freezing induced indiscriminate killing. Home collection and freezing of sputum will enable frequent and convenient assessment of P. aeruginosa population dynamics in CF.
铜绿假单胞菌在囊性纤维化（CF）气道中会出现显著的群体异质性。这些群体的变化与临床状况之间的关系尚不清楚。为了便于理解这一点，需要对这个群落进行频繁采样。为了确定在家中采集和储存痰液是否可行，我们从10名患者那里采集了痰液。痰液样本被分成两份，一半立即在麦康凯琼脂上进行处理，另一半在家庭冰箱中冷冻一周后进行评估。从每个样本中，对88株分离菌进行了抗生素敏感性和毒力因子产生情况的评估。冷冻导致铜绿假单胞菌数量下降了10CFU/ml。然而，在1760株分离菌中，未观察到抗生素敏感性或毒力因子方面的一致差异，这表明冷冻导致了无差别杀伤。在家中采集和冷冻痰液将能够对CF患者气道中的铜绿假单胞菌群体动态进行频繁且便捷的评估。
