静脉注射布洛芬和氯诺昔康对后肢缺血再灌注损伤大鼠红细胞变形性的影响。

Effects of intravenous ibuprofen and lornoxicam on erythrocyte deformability in rats undergoing hind limb ischemia reperfusion injury.

作者信息

Sivgin V, Kucuk A, Comu F M, Kosem B, Kartal S, Turgut H C, Arpaci H, Aydin M E, Koc D S, Ozer A, Arslan M, Alkan M

出版信息

Bratisl Lek Listy. 2016;117(12):722-725. doi: 10.4149/BLL_2016_138.

DOI:10.4149/BLL_2016_138

PMID:28127969

Abstract

BACKGROUND AND AIM

Acute hind limb ischemia reperfusion (I/R) injury is a common consequence of abdominal aorta cross‑clamping during aortic surgery. Erythrocyte deformability is affected by I/R process and may lead to increased tissue and organ injury. Lornoxicam and intravenous ibuprofen are becoming commonly used as non-steroidal anti-inflammatory drugs (NSAID) for postoperative analgesia. In this study, we aimed to investigate the effects of lornoxicam (2 mg/kg iv) and intravenous ibuprofen (30 mg/kg iv) on erythrocyte deformability in I/R model in rats.

MATERIALS AND METHODS

Four study groups, each containing 6 Wistar rats were created. Laparotomy was performed in all groups under general anesthesia with ketamine and xylazine. In all groups except sham group, ischemia and reperfusion were achieved by clamping and declamping the infrarenal abdominal aorta for 120 minutes. Rats in Group IR+L received intravenous infusion of lornoxicam (2 mg/kg) while rats in Group IR+I received intravenous infusion of ibubrofen (30 mg/kg) following 2 hours of ischemic period. At the end of reperfusion period, erythrocyte packs were prepared from heparinized blood samples. Erythrocyte suspensions with hematocrit at a concentration of 5% in a phosphate‑buffered saline (PBS) were used in order to perform deformability measurements. The value of p<0.05 was considered statistically significant.

RESULTS

Relative resistance has increased in ischemia reperfusion group when compared to control group (p < 0.0001). Lornoxicam or ibuprofen intravenous treatments did not change the erythrocyte deformability during ischemia reperfusion period in rats (p=0.851, p=0.690).

CONCLUSION

Intravenous ibuprofen or lornoxicam administrations during ischemia reperfusion period in rats have no negative effect on erythrocyte deformability. The findings of the study should be supported with more detailed and extensive clinical/experimental studies in the future (Fig. 1, Ref. 18).

摘要

背景与目的

急性后肢缺血再灌注（I/R）损伤是主动脉手术中腹主动脉交叉钳夹的常见后果。红细胞变形性受I/R过程影响，可能导致组织和器官损伤加重。氯诺昔康和静脉注射布洛芬正逐渐成为常用的非甾体抗炎药（NSAID）用于术后镇痛。在本研究中，我们旨在探讨氯诺昔康（2 mg/kg静脉注射）和静脉注射布洛芬（30 mg/kg静脉注射）对大鼠I/R模型中红细胞变形性的影响。

材料与方法

创建四个研究组，每组包含6只Wistar大鼠。所有组均在氯胺酮和赛拉嗪全身麻醉下进行剖腹手术。除假手术组外，所有组通过钳夹和松开肾下腹主动脉120分钟实现缺血和再灌注。IR+L组大鼠在缺血2小时后接受静脉输注氯诺昔康（2 mg/kg），而IR+I组大鼠接受静脉输注布洛芬（30 mg/kg）。在再灌注期结束时，从肝素化血样中制备红细胞包。使用在磷酸盐缓冲盐水（PBS）中血细胞比容浓度为5%的红细胞悬液进行变形性测量。p<0.05的值被认为具有统计学意义。