Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH, 43210, USA.
Angew Chem Int Ed Engl. 2017 Mar 1;56(10):2763-2766. doi: 10.1002/anie.201612079. Epub 2017 Jan 27.
Catalysts that promote carbohydrate degradation have a wide range of potential applications, but the use of either enzyme glycosidases or small-molecule catalysts in biological systems raises significant challenges. Herein, we demonstrate a novel strategy for the design of synthetic agents that mimic natural glycosidases and address current problems for biological use. This strategy is illustrated by application to the development of potential blood substitutes for the rare Bombay blood type that is characterized by a deficiency of H2 antigen. Metallopeptides with 16 to 20 amino acids were constructed as artificial fucosidases that exhibit selective carbohydrate cleavage reactivity toward l-fucose over d-glucose. Selective fucose cleavage from the H2-antigen saccharide enables efficient removal of H2 antigen from erythrocytes and thereby accomplishes the conversion of regular human type-O blood into a potential blood substitute for the rare Bombay blood type.
促进碳水化合物降解的催化剂具有广泛的潜在应用，但在生物系统中使用酶糖苷酶或小分子催化剂会带来重大挑战。在此，我们展示了一种设计模拟天然糖苷酶的合成试剂的新策略，并解决了生物应用中的当前问题。该策略通过应用于开发罕见的孟买血型的潜在血液替代品得到了说明，这种血型的特征是缺乏 H2 抗原。构建了具有 16 至 20 个氨基酸的金属肽作为人工岩藻糖苷酶，其对 l-岩藻糖相对于 d-葡萄糖表现出选择性的碳水化合物切割反应性。从 H2 抗原糖中选择性切割岩藻糖可有效地从红细胞中去除 H2 抗原，从而将常规的人类 O 型血转化为罕见的孟买血型的潜在血液替代品。
