新型益生菌干酪乳杆菌来源的α-L-岩藻糖苷酶对天然岩藻糖基化低聚糖的利用
Utilization of natural fucosylated oligosaccharides by three novel alpha-L-fucosidases from a probiotic Lactobacillus casei strain.
机构信息
Laboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, Paterna, Valencia, Spain.
出版信息
Appl Environ Microbiol. 2011 Jan;77(2):703-5. doi: 10.1128/AEM.01906-10. Epub 2010 Nov 19.
Abstract
Three putative α-L-fucosidases encoded in the Lactobacillus casei BL23 genome were cloned and purified. The proteins displayed different abilities to hydrolyze natural fucosyloligosaccharides like 2'-fucosyllactose, H antigen disaccharide, H antigen type II trisaccharide, and 3'-, 4'-, and 6'-fucosyl-GlcNAc. This indicated a possible role in the utilization of oligosaccharides present in human milk and intestinal mucosa.
摘要
从干酪乳杆菌 BL23 基因组中克隆并纯化了三种假定的α-L-岩藻糖苷酶。这些蛋白对天然岩藻糖基低聚糖（如 2'-岩藻糖基乳糖、H 抗原二糖、H 抗原 II 型三糖以及 3'、4'、6'-岩藻糖基-GlcNAc）的水解能力不同。这表明它们可能在利用人乳和肠道黏膜中存在的寡糖方面发挥作用。
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