Yu Yongbo, Fu Libing, Wang Shen, Jin Yaqiong, Han Shujing, Chu Ping, Lu Jie, Guo Yongli, He Lejian, Ni Xin
Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing, China.
Clinical Diagnostic Center, 302nd Hospital of the People's Liberation Army, Beijing, China.
Growth Horm IGF Res. 2017 Apr;33:17-22. doi: 10.1016/j.ghir.2017.01.002. Epub 2017 Jan 13.
Many efforts have been made to address involvement of the insulin-like growth-factor (IGF) pathway in rhabdomyosarcoma (RMS) pathogenesis, but the actual role of IGF in RMS is still controversial.
To investigate the implications of IGF2, IGFBP2 and p63 in RMS, and further explored their potential interaction.
A total of 114 specimens of RMS along with clinic-pathologic characteristics were collected from the year of 2003 to 2013. Protein abundance was detected by immunohistochemical staining, potential relationships between protein levels and clinic-pathological parameters were applied using correlation analysis.
The results showed positive correlation between IGFBP2 and p63 (r=0.271, p=0.003), suggesting that the interaction of IGFBP2 and p63 might account for the pathogenesis of RMS. In the subtype analysis, positive correlation was still found in embryonal rhabdomyosarcoma (ERMS, r=0.214, p=0.034) and alveolar rhabdomyosarcoma (ARMS, r=0.498, p=0.048). By focusing on the interaction of IGF pathway and p63, our results reveal additional signs to elucidate difference of pathogenesis and severity between ERMS and ARMS.
The present study provides novel evidence to elucidate RMS pathogenesis and may be beneficial to clinical diagnosis and therapy for RMS.
为探讨胰岛素样生长因子（IGF）通路在横纹肌肉瘤（RMS）发病机制中的作用，已开展了多项研究，但IGF在RMS中的实际作用仍存在争议。
研究IGF2、IGFBP2和p63在RMS中的意义，并进一步探讨它们之间的潜在相互作用。
收集2003年至2013年期间114例RMS标本及其临床病理特征。采用免疫组织化学染色检测蛋白丰度，应用相关性分析研究蛋白水平与临床病理参数之间的潜在关系。
结果显示IGFBP2与p63呈正相关（r=0.271，p=0.003），提示IGFBP2与p63的相互作用可能参与RMS的发病机制。在亚型分析中，胚胎型横纹肌肉瘤（ERMS，r=0.214，p=0.034）和腺泡状横纹肌肉瘤（ARMS，r=0.498，p=0.048）中仍存在正相关。通过关注IGF通路与p63的相互作用，我们的结果揭示了更多迹象，以阐明ERMS和ARMS在发病机制和严重程度上的差异。
本研究为阐明RMS发病机制提供了新的证据，可能有助于RMS的临床诊断和治疗。
