Han Ling, Sun Jing, Lu Chuan-Jian, Zhao Rui-Zhi, Lu Yue, Lin Han-Jie, Wei Jian-An
The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangdong 510006, China.
Dermatology Department, Guangdong Provincial Hospital of Chinese Medicine, Guangdong 510120, China.
Int Immunopharmacol. 2017 Mar;44:226-233. doi: 10.1016/j.intimp.2017.01.023. Epub 2017 Jan 25.
Psoriasis is a common chronic inflammatory disease in which T-helper 1(Th1) and T-helper 17(Th17) cells play an important role in its pathology. Formula PSORI-CM01 was a novel formulated Chinese medicine used for psoriasis therapy. It had been demonstrated previously that PSORI-CM01 and serum contained Formula PSORI-CM01 (PCM01CS) could improve psoriasis by inhibiting the epithelial hyperplasia, how PSORI-CM01 affects inflammatory cytokine and chemokine in dermis is still unknown. In this study we found PSORI-CM01 pre-treated 3days before IMQ painting could ameliorated IMQ-induced mice skin lesion as PASI score was apparently reduced. Th1 related cytokine IFN-γ and Th17 related cytokine IL-17/IL-22 was used to induce inflammatory models on human keratinocyte cell line HaCaT in vitro, respectively. PCM01CS significantly reduced IFN-γ induced mRNA expression of IL-6, IL-12 and CXCL-10, reduced IL-6 and CXCL-10 release into HaCaT supernatant. 20ng/ml IL-17/IL-22 co-stimulation significantly upregulated expression of IL-6, IL-8 and CCL20 mRNA expression in HaCaT cells, PCM01CS significantly inhibit these cytokines expression both in mRNA and in protein levels. Finally, PCM01CS could obviously inhibit nuclear NF-κB p65 expression which activated by IFN-γ and IL-17/IL-22 stimulation. Thus, our new findings reveal that Formula PSORI-CM01 may possess therapeutic action on psoriasis by inhibiting inflammatory within skin environments.
银屑病是一种常见的慢性炎症性疾病，其中辅助性T细胞1（Th1）和辅助性T细胞17（Th17）在其病理过程中起重要作用。PSORI-CM01配方是一种用于治疗银屑病的新型中药配方。先前已经证明，PSORI-CM01及含PSORI-CM01的血清（PCM01CS）可通过抑制上皮增生来改善银屑病，但PSORI-CM01如何影响真皮中的炎性细胞因子和趋化因子仍不清楚。在本研究中，我们发现，在涂抹咪喹莫特前3天用PSORI-CM01预处理可改善咪喹莫特诱导的小鼠皮肤病变，因为银屑病面积和严重程度指数（PASI）评分明显降低。分别使用Th1相关细胞因子干扰素-γ（IFN-γ）和Th17相关细胞因子白细胞介素-17（IL-17）/白细胞介素-22（IL-22）在体外人角质形成细胞系HaCaT上诱导炎症模型。PCM01CS显著降低IFN-γ诱导的白细胞介素-6（IL-6）、白细胞介素-12（IL-12）和CXC趋化因子配体10（CXCL-10）的mRNA表达，减少IL-6和CXCL-10释放到HaCaT细胞上清液中。20纳克/毫升IL-17/IL-22共刺激显著上调HaCaT细胞中IL-6、白细胞介素-8（IL-8）和CC趋化因子配体20（CCL20）的mRNA表达，PCM01CS在mRNA和蛋白质水平上均显著抑制这些细胞因子的表达。最后，PCM01CS可明显抑制由IFN-γ和IL-17/IL-22刺激激活的核转录因子κB p65（NF-κB p65）的表达。因此，我们的新发现表明，PSORI-CM01配方可能通过抑制皮肤内环境中的炎症对银屑病具有治疗作用。
