在链脲佐菌素诱导的1型糖尿病小鼠模型中，骆驼乳清蛋白通过Bcl-XL/Bax表达改善淋巴器官中的氧化应激和组织病理学改变。
Camel whey protein improves oxidative stress and histopathological alterations in lymphoid organs through Bcl-XL/Bax expression in a streptozotocin-induced type 1 diabetic mouse model.
作者信息
Sayed Leila H, Badr Gamal, Omar Hossam M, Abd El-Rahim Ali M, Mahmoud Mohamed H
机构信息
Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt; Laboratory of Immunology and Molecular Physiology, Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt; Laboratory of Physiology, Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt; Laboratory of Immunology and Molecular Physiology, Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt.
出版信息
Biomed Pharmacother. 2017 Apr;88:542-552. doi: 10.1016/j.biopha.2017.01.076. Epub 2017 Jan 24.
Type I diabetes (T1D) is a characterized by the inflammation of pancreatic islets and destruction of β cells. Long and persistent uncontrolled diabetes tends to degenerate the immune system and increase the incidence of infections in diabetic individuals. Most serious diabetic complications are mediated by the free radicals, which damage multiple cellular components through direct effects of the cell cycle regulatory proteins. Camel whey protein (CWP) has antioxidant activity and decreases the effects of free radicals. However, the effects of CWP on lymphoid organs have not been studied in the context of diabetes. Therefore, the present study was designed to investigate the dietary influence of CWP supplementation on the lymphoid organs in streptozotocin (STZ)-induced type 1 diabetic mouse model. Three experimental groups were used: non diabetic control mice, diabetic mice, and diabetic mice treated with CWP. Induction of diabetes was associated with a marked reduction in glutathione (GSH) levels; decreased activities of GSH peroxidase (GSH Px), manganese superoxide dismutase (MnSOD) and catalase; increased reactive oxygen species (ROS) levels and iNOS activity in plasma and lymphoid organs. Furthermore, diabetic mice exhibited alterations in the expression of Bax and Bcl-XL, and subsequently pathological alterations in the architecture of the bone marrow, pancreas, thymus, and spleen. Interestingly, treatment of diabetic mice with CWP robustly restored glucose, insulin, GSH, and ROS levels and the activities of GSH Px, MnSOD, catalase and iNOS. Additionally, supplementation of diabetic mice with CWP improvement in the architecture of lymphoid tissues and rescued from apoptosis through direct effects on the Bax and Bcl-XL proteins. These data revealed the therapeutic potential of CWP against diabetic complications mediated damages of lymphoid organs.
1型糖尿病（T1D）的特征是胰岛炎症和β细胞破坏。长期持续的糖尿病控制不佳往往会使免疫系统退化，并增加糖尿病患者感染的发生率。最严重的糖尿病并发症是由自由基介导的，自由基通过细胞周期调节蛋白的直接作用损害多种细胞成分。骆驼乳清蛋白（CWP）具有抗氧化活性，可降低自由基的影响。然而，在糖尿病背景下，尚未研究CWP对淋巴器官的影响。因此，本研究旨在探讨补充CWP对链脲佐菌素（STZ）诱导的1型糖尿病小鼠模型中淋巴器官的饮食影响。使用了三个实验组：非糖尿病对照小鼠、糖尿病小鼠和用CWP治疗的糖尿病小鼠。糖尿病的诱导与谷胱甘肽（GSH）水平显著降低、GSH过氧化物酶（GSH Px）、锰超氧化物歧化酶（MnSOD）和过氧化氢酶活性降低、血浆和淋巴器官中活性氧（ROS）水平和诱导型一氧化氮合酶（iNOS）活性增加有关。此外，糖尿病小鼠在Bax和Bcl-XL的表达上表现出改变，随后在骨髓、胰腺、胸腺和脾脏的结构上出现病理改变。有趣的是，用CWP治疗糖尿病小鼠可有力地恢复血糖、胰岛素、GSH和ROS水平以及GSH Px、MnSOD、过氧化氢酶和iNOS的活性。此外，给糖尿病小鼠补充CWP可改善淋巴组织的结构，并通过对Bax和Bcl-XL蛋白的直接作用使其免于凋亡。这些数据揭示了CWP对糖尿病并发症介导的淋巴器官损伤的治疗潜力。
Zotero 插件