Zhao Liangjie, Chenoweth Erica L, Liu Qigen
a The Key Laboratory of Aquatic Genetic Resources and Utilization (AGRU) of the Ministry of Agriculture , Shanghai Ocean University , Shanghai , P. R. China.
b Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang Agriculture and Forestry University , Xinyang , P. R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Mar;29(2):303-311. doi: 10.1080/24701394.2016.1278533. Epub 2017 Jan 27.
In order to understand the influence of human activities such as habitat fragmentation on freshwater fish population evolution, we investigated and compared the genetic diversity and phylogeography of Sinibrama macrops populations in the Oujiang River and Ling River. Mitochondrial control region sequences (D-loop region) of 131 specimens from six populations were obtained and analyzed. The diversity of main stream in the Ou River was lower than that in Ling River. Changtan population showed the lowest diversity (H = 0.646 ± 0.077; π = 0.00060 ± 0.00820). Pairwise F, gene flow (Nm), and genetic distance (Da) indicated that Longquan and Changtan significantly differentiate from other populations. Nested clade phylogeographical analysis (NCPA) showed some clades and total cladogram experienced isolation by distance. In conclusion, the populations from severely fragmented Ou River have the lower diversity and more intense differentiation than that from the mainstream of Ling River, Changtan population present the lowest diversity and were isolated by the dam construction.
为了解栖息地破碎化等人类活动对淡水鱼类种群进化的影响，我们调查并比较了瓯江和灵江大眼华鳊种群的遗传多样性和系统地理学。获得并分析了来自六个种群的131个样本的线粒体控制区序列（D-loop区）。瓯江干流的多样性低于灵江。长潭种群的多样性最低（H = 0.646 ± 0.077；π = 0.00060 ± 0.00820）。成对F、基因流（Nm）和遗传距离（Da）表明龙泉和长潭与其他种群有显著差异。嵌套支系系统地理学分析（NCPA）表明一些支系和总图经历了距离隔离。总之，与灵江干流相比，严重破碎化的瓯江种群多样性较低，分化更强烈，长潭种群多样性最低，且受到大坝建设的隔离。
