使用多重聚合酶链反应快速鉴别bla、bla和bla。
Rapid discrimination of bla, bla, and bla using a multiplex PCR.
作者信息
Kayama Shizuo, Ohge Hiroki, Sugai Motoyuki
机构信息
Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan; Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Japan.
Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan; Department of Infectious Diseases, Hiroshima University Hospital, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
出版信息
J Microbiol Methods. 2017 Apr;135:8-10. doi: 10.1016/j.mimet.2017.01.013. Epub 2017 Jan 24.
Stealth-type carbapenem-resistant Enterobacteriaceae that are resistant to almost all β-lactams except imipenem is emerging in Japan. This resistance is mediated by specific variants of the metallo-β-lactamases (bla or bla) that differs by one amino acid from the common variant bla. We developed an amplification refractory mutation system (ARMS) PCR assay enabling rapid, sequence independent, identification of these variants.
在日本，一种对除亚胺培南外几乎所有β-内酰胺类药物耐药的隐匿型耐碳青霉烯类肠杆菌科细菌正在出现。这种耐药性由金属β-内酰胺酶（bla或bla）的特定变体介导，这些变体与常见变体bla在一个氨基酸上存在差异。我们开发了一种扩增阻滞突变系统（ARMS）PCR检测方法，能够快速、不依赖序列地鉴定这些变体。
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