壳聚糖包被的脂质核纳米囊对牛精子细胞的影响。
Effects of chitosan-coated lipid-core nanocapsules on bovine sperm cells.
作者信息
de Castro Jorge Silva Arthur, Remirão Mariana Härter, Lucas Caroline Gomes, Domingues William Borges, Silveira Tony, Paschoal Julia Dame, Jornada Denise S, Corcine Carine Dahl, Junior Antonio Sergio Varela, Prado Willian A, Campos Vinicius Farias, Seixas Fabiana Kömmling, Guterres Silvia S, Pohlmann Adriana R, Collares Tiago
机构信息
Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Laboratório de Biotecnologia do Câncer, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas 96010-610, RS, Brazil.
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, RS, Brazil.
出版信息
Toxicol In Vitro. 2017 Apr;40:214-222. doi: 10.1016/j.tiv.2017.01.017. Epub 2017 Jan 24.
Toxicology studies have a pivotal role for selection of new nanosystems. As lipid-core nanocapsules (LNC) rise as a potential system not only for drug delivery but also for immunotherapy and gene therapy, the demand for models of toxic screening increases, and sperm arises as a promising model due to the easiness to evaluate its viability parameters. LNCs were coated with chitosan, chitosan-coated lipid-core nanocapsules (LNC-CS), in order to modify the nanocapsule surface. We evaluated the toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations (2.5%, 5%, 10%, 20%, 40% and 80%) (v/v) and periods of exposure (0h and 1h). CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects. The results corroborated with previous studies indicating that there is no toxicity from LNCs and LNC-CS below 40% (v/v) concentration.
毒理学研究在新型纳米系统的筛选中起着关键作用。随着脂质核纳米胶囊（LNC）不仅作为药物递送的潜在系统，而且作为免疫疗法和基因疗法的潜在系统兴起，对毒性筛选模型的需求增加，而精子因其易于评估其活力参数而成为一种有前景的模型。LNC用壳聚糖进行了包被，即壳聚糖包被的脂质核纳米胶囊（LNC-CS），以修饰纳米胶囊表面。我们评估了LNC和LNC-CS在与不同浓度（2.5%、5%、10%、20%、40%和80%）（v/v）的牛精子孵育以及不同暴露时间（0小时和1小时）后的毒性。进行了计算机辅助精子分析（CASA）参数和流式细胞术检测以评估LNC和LNC-CS的影响。结果与先前的研究一致，表明浓度低于40%（v/v）时，LNC和LNC-CS没有毒性。
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