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Direct demonstration of termination signals for RNA polymerase II from the sea urchin H2A histone gene.

作者信息

Briggs D, Jackson D, Whitelaw E, Proudfoot N J

机构信息

Sir William Dunn School of Pathology, University of Oxford, UK.

出版信息

Nucleic Acids Res. 1989 Oct 25;17(20):8061-71. doi: 10.1093/nar/17.20.8061.

DOI:10.1093/nar/17.20.8061
PMID:2813057
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334947/
Abstract

Previous studies [1,2] suggested but did not prove that the sea urchin H2A histone gene possesses strong transcriptional termination signals close to, but separate from, the 3' processing signals. In this study we have demonstrated by two independent approaches that these sequences elicit authentic transcriptional termination. First we show by nuclear run off analysis that nascent transcription terminates in the immediate 3' flanking region of the H2A gene, in an A-rich region. Second we show that these termination signals prevent transcriptional read through when placed in the intron of a globin gene. The intronic position of the termination signal rules out any effect on steady state mRNA levels. We have therefore defined DNA sequences which act as a transcription terminator when placed in heterologous RNA polymerase II genes.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cb2/334947/9e5b4f2f1166/nar00137-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cb2/334947/93d0bbd6faec/nar00137-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cb2/334947/9e5b4f2f1166/nar00137-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cb2/334947/93d0bbd6faec/nar00137-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cb2/334947/9e5b4f2f1166/nar00137-0049-a.jpg

相似文献

1
Direct demonstration of termination signals for RNA polymerase II from the sea urchin H2A histone gene.
Nucleic Acids Res. 1989 Oct 25;17(20):8061-71. doi: 10.1093/nar/17.20.8061.
2
Tripartite sequences within and 3' to the sea urchin H2A histone gene display properties associated with a transcriptional termination process.海胆H2A组蛋白基因内部及其3'端的三联体序列表现出与转录终止过程相关的特性。
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3
A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene.在小鼠β珠蛋白基因中，高效终止RNA聚合酶II转录需要一个聚腺苷酸（poly(A)）添加位点和一个下游终止区域。
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5
Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences.海胆组蛋白mRNA末端位于假定调控序列下游的基因区域。
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本文引用的文献

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The organization and expression of histone gene families.组蛋白基因家族的组织与表达
Cell. 1981 Aug;25(2):301-13. doi: 10.1016/0092-8674(81)90048-9.
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3' editing of mRNAs: sequence requirements and involvement of a 60-nucleotide RNA in maturation of histone mRNA precursors.信使核糖核酸的3'端编辑：序列要求及一段60个核苷酸的核糖核酸在组蛋白信使核糖核酸前体成熟过程中的作用
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Recently transposed Alu repeats result from multiple source genes.最近转座的Alu重复序列源自多个源基因。
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Mol Cell Biol. 1991 Sep;11(9):4599-615. doi: 10.1128/mcb.11.9.4599-4615.1991.
禽逆转录病毒中的转录干扰——对白血病发生的启动子插入模型的启示
Nature. 1984;307(5948):241-5. doi: 10.1038/307241a0.
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New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
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DNA sequences necessary for transcription of the rabbit beta-globin gene in vivo.兔β-珠蛋白基因在体内转录所需的DNA序列。
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6
Transcriptional regulation of hemoglobin switching in chicken embryos.鸡胚中血红蛋白转换的转录调控。
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A general method for preparing chromatin containing intact DNA.一种制备含有完整DNA的染色质的通用方法。
EMBO J. 1985 Apr;4(4):913-8. doi: 10.1002/j.1460-2075.1985.tb03718.x.
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Formation of the 3' end of U1 snRNA requires compatible snRNA promoter elements.U1小核仁核糖核酸（snRNA）3'端的形成需要兼容的小核仁核糖核酸启动子元件。
Cell. 1986 Oct 24;47(2):249-58. doi: 10.1016/0092-8674(86)90447-2.
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Transcriptional interference and termination between duplicated alpha-globin gene constructs suggests a novel mechanism for gene regulation.重复的α-珠蛋白基因构建体之间的转录干扰和终止提示了一种新的基因调控机制。
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A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.GM-CSF信使核糖核酸3'非翻译区的一段保守AU序列介导选择性信使核糖核酸降解。
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