Cappuccio Javier, Dibarbora Marina, Lozada Inés, Quiroga Alejandra, Olivera Valeria, Dángelo Marta, Pérez Estefanía, Barrales Hernán, Perfumo Carlos, Pereda Ariel, Pérez Daniel R
Instituto de Virología, CICVyA, INTA, Nicolas Repetto y de los Reseros s/n, 1686, Hurlingham, Buenos Aires, Argentina; CONICET, Av. Rivadavia 1917, C1033AAJ, CABA, Argentina.
Instituto de Virología, CICVyA, INTA, Nicolas Repetto y de los Reseros s/n, 1686, Hurlingham, Buenos Aires, Argentina; CONICET, Av. Rivadavia 1917, C1033AAJ, CABA, Argentina.
Comp Immunol Microbiol Infect Dis. 2017 Feb;50:110-115. doi: 10.1016/j.cimid.2016.12.005. Epub 2016 Dec 26.
Swine farms provide a dynamic environment for the evolution of influenza A viruses (IAVs). The present report shows the results of a surveillance effort of IAV infection in one commercial swine farm in Argentina. Two cross-sectional serological and virological studies (n=480) were carried out in 2011 and 2012. Virus shedding was detected in nasal samples from pigs from ages 7, 21 and 42-days old. More than 90% of sows and gilts but less than 40% of 21-days old piglets had antibodies against IAV. In addition, IAV was detected in 8/17 nasal swabs and 10/15 lung samples taken from necropsied pigs. A subset of these samples was further processed for virus isolation resulting in 6 viruses of the H1N2 subtype (δ2 cluster). Pathological studies revealed an association between suppurative bronchopneumonia and necrotizing bronchiolitis with IAV positive samples. Statistical analyses showed that the degree of lesions in bronchi, bronchiole, and alveoli was higher in lungs positive to IAV. The results of this study depict the relevance of continuing long-term active surveillance of IAV in swine populations to establish IAV evolution relevant to swine and humans.
养猪场为甲型流感病毒（IAV）的进化提供了一个动态环境。本报告展示了在阿根廷一个商业养猪场对IAV感染进行监测的结果。2011年和2012年开展了两项横断面血清学和病毒学研究（n = 480）。在7日龄、21日龄和42日龄仔猪的鼻腔样本中检测到病毒 shedding 。超过90%的母猪和后备母猪具有抗IAV抗体，但21日龄仔猪中该比例不到40%。此外，在对病死猪采集的17份鼻腔拭子中的8份以及15份肺样本中的10份中检测到了IAV。对这些样本的一个子集进一步进行病毒分离，得到了6株H1N2亚型（δ2簇）病毒。病理学研究揭示了化脓性支气管肺炎和坏死性细支气管炎与IAV阳性样本之间的关联。统计分析表明，IAV阳性的肺组织中支气管、细支气管和肺泡的病变程度更高。本研究结果表明，持续对猪群中的IAV进行长期主动监测对于确定与猪和人类相关的IAV进化具有重要意义。
