Rodríguez-Castelán J, Méndez-Tepepa M, Carrillo-Portillo Y, Anaya-Hernández A, Rodríguez-Antolín J, Zambrano E, Castelán F, Cuevas-Romero E
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, TLAX, Mexico.
Departamento de Biología de la Reproducción, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
Biomed Res Int. 2017;2017:3795950. doi: 10.1155/2017/3795950. Epub 2017 Jan 4.
Ovarian failure is related to dyslipidemias and inflammation, as well as to hypertrophy and dysfunction of the visceral adipose tissue (VAT). Although hypothyroidism has been associated with obesity, dyslipidemias, and inflammation in humans and animals, its influence on the characteristics of ovarian follicles in adulthood is scarcely known. Control and hypothyroid rabbits were used to analyze the ovarian follicles, expression of aromatase in the ovary, serum concentration of lipids, leptin, and uric acid, size of adipocytes, and infiltration of macrophages in the periovarian VAT. Hypothyroidism did not affect the percentage of functional or atretic follicles. However, it reduced the size of primary, secondary, and tertiary follicles considered as large and the expression of aromatase in the ovary. This effect was associated with high serum concentrations of total cholesterol and low-density lipoprotein cholesterol (LDL-C). In addition, hypothyroidism induced hypertrophy of adipocytes and a major infiltration of CD68+ macrophages into the periovarian VAT. Our results suggest that the reduced size of ovarian follicles promoted by hypothyroidism could be associated with dyslipidemias, hypertrophy, and inflammation of the periovarian VAT. Present findings may be useful to understand the influence of hypothyroidism in the ovary function in adulthood.
卵巢功能衰竭与血脂异常、炎症以及内脏脂肪组织（VAT）的肥大和功能障碍有关。尽管甲状腺功能减退症在人类和动物中与肥胖、血脂异常和炎症相关，但其对成年期卵巢卵泡特征的影响却鲜为人知。本研究使用对照兔和甲状腺功能减退兔来分析卵巢卵泡、卵巢中芳香化酶的表达、血脂、瘦素和尿酸的血清浓度、脂肪细胞大小以及卵巢周围VAT中巨噬细胞的浸润情况。甲状腺功能减退症并未影响功能性或闭锁卵泡的百分比。然而，它减小了被视为大卵泡的初级、次级和三级卵泡的大小，并降低了卵巢中芳香化酶的表达。这种影响与血清总胆固醇和低密度脂蛋白胆固醇（LDL-C）的高浓度有关。此外，甲状腺功能减退症导致脂肪细胞肥大以及CD68 +巨噬细胞大量浸润到卵巢周围VAT中。我们的结果表明，甲状腺功能减退症促使卵巢卵泡变小可能与卵巢周围VAT的血脂异常、肥大和炎症有关。目前的研究结果可能有助于理解甲状腺功能减退症对成年期卵巢功能的影响。
