泽尼特类风湿关节炎评估，一种用于检测抗细胞抗体的固相化学发光免疫分析方法。

Zenit RA evaluation, a solid-phase chemiluminescence immunoassay for detection of anti-cellular antibodies.

作者信息

Melguizo Madrid Enrique, González-Rodríguez Concepción, Ávila-García María de Gracia, Arrobas-Velilla Teresa, Fernández-Riejos Patricia

机构信息

UGC Clinical Biochemistry, Virgen Macarena University Hospital, Dr Fedriani 3 Avenue - 41009 Seville, Spain.

出版信息

Bioanalysis. 2017 Mar;9(5):435-445. doi: 10.4155/bio-2016-0252. Epub 2017 Jan 30.

DOI:10.4155/bio-2016-0252

PMID:28134563

Abstract

AIM

The objective was to compare Zenit RA chemiluminescent immunoassay (CLIA) from Menarini Diagnostics and ELISA from INOVA Diagnostics for the presence of specific anti-Ro/SS-A, anti-La/SS-B, anti-U1snRNP, anti-Sm, anti-Scl-70, anti-Jo-1 antibodies. Results/methodology: We studied 501 samples (178 connective autoimmune disease, 150 other autoimmune or inflammatory disease and 173 other disease or healthy). All samples were analyzed using CLIA and ELISA. The Kappa agreement was excellent for anti-SSA/Ro (0.864), good for anti-SSB/La (0.735), anti-Scl-70 (0.685) and ENA-screening (0.778), moderate for anti-RNP (0.563) and bad for anti-Sm (0.266) and anti-Jo-1 (0.243). Different combination of cut-off improved the specificity and agreement.

CONCLUSION

Zenit RA CLIA for detecting autoantibodies, provides a simple, useful and accurate tool.

摘要

目的

比较美纳里尼诊断公司的泽尼特类风湿关节炎化学发光免疫分析法（CLIA）和英诺华诊断公司的酶联免疫吸附测定法（ELISA）检测特异性抗Ro/SS - A、抗La/SS - B、抗U1snRNP、抗Sm、抗Scl - 70、抗Jo - 1抗体的情况。结果/方法：我们研究了501份样本（178份结缔组织自身免疫性疾病样本、150份其他自身免疫性或炎性疾病样本以及173份其他疾病或健康样本）。所有样本均采用CLIA和ELISA进行分析。抗SSA/Ro的kappa一致性极佳（0.864），抗SSB/La（0.735）、抗Scl - 70（0.685）和可提取核抗原筛查（0.778）的一致性良好，抗RNP（0.563）的一致性中等，抗Sm（0.266）和抗Jo - 1（0.243）的一致性较差。不同的临界值组合提高了特异性和一致性。