Zhang Xiaona, Niu Jiahua, Zhang Xiaoting, Xiao Rui, Lu Minghua, Cai Zongwei
Institute of Environmental and Analysis Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China.
Institute of Environmental and Analysis Science, School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Mar 1;1046:58-64. doi: 10.1016/j.jchromb.2017.01.004. Epub 2017 Jan 5.
In this research, a modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method based on graphene oxide@SiO(SiO@GO) nanocomposite as adsorbent of dispersive solid-phase extraction (dSPE) combined with high performance liquid chromatography (HPLC) for the analysis of four plant hormones in different plants was established. The as-prepared SiO@GO was characterized by scanning electron microscopy, transmission electron microscopy and infrared spectroscopy. The experimental conditions for dSPE, including the ratio of material to liquid, pH of sample, adsorption and desorption time, desorption temperature as well as desorption solution, were investigated. The detection limits for the analysis of indole-3-acetic acid, indole-3-butyric acid, 1-naphthylacetic acid and abscisic acid were achieved below 0.05μgmL. The established method was applied to the analysis of the plant hormones in fruits, vegetables and other food samples. The obtained results indicated that the method was sensitive, accurate, convenient and quick, which provided an alternative analytical approach for plant hormones in complex matrices.
本研究建立了一种基于氧化石墨烯@SiO（SiO@GO）纳米复合材料作为分散固相萃取（dSPE）吸附剂，结合高效液相色谱（HPLC）分析不同植物中四种植物激素的改进的快速、简便、廉价、高效、耐用且安全（QuEChERS）方法。通过扫描电子显微镜、透射电子显微镜和红外光谱对所制备的SiO@GO进行了表征。研究了dSPE的实验条件，包括料液比、样品pH值、吸附和解吸时间、解吸温度以及解吸溶液。吲哚-3-乙酸、吲哚-3-丁酸、1-萘乙酸和脱落酸的检测限均达到0.05μg/mL以下。所建立的方法应用于水果、蔬菜及其他食品样品中植物激素的分析。所得结果表明该方法灵敏、准确、方便且快速，为复杂基质中植物激素提供了一种替代分析方法。
