Urrego R, Bernal-Ulloa S M, Chavarría N A, Herrera-Puerta E, Lucas-Hahn A, Herrmann D, Winkler S, Pache D, Niemann H, Rodriguez-Osorio N
Grupo INCA-CES,Facultad de Medicina Veterinaria y Zootecnia,Universidad CES,Calle 10 A No. 22-04 MedellínColombia.
Institute of Farm Animal Genetics,Friedrich-Loeffler-Institut (FLI),Höltystraße 10,Mariensee,31535 Neustadt,Germany.
Zygote. 2017 Apr;25(2):131-140. doi: 10.1017/S096719941600040X. Epub 2017 Jan 31.
Bovine embryos produced in vivo and in vitro differ with respect to molecular profiles, including epigenetic marks and gene expression profiles. This study investigated the CpG methylation status in bovine testis satellite I (BTS) and Bos taurus alpha satellite I (BTαS) DNA sequences, and concomitantly the relative abundance of transcripts, critically involved in DNA methylation (DNMT1 and DNMT3A), growth and development (IGF2R) and pluripotency (POU5F1) in Bos indicus embryos produced in vitro or in vivo. Results revealed that methylation of BTS were higher (P < 0.05) in embryos produced in vitro compared with their in vivo produced counterparts, while the methylation status of BTαS was similar in both groups. There were no significant differences in transcript abundance for DNMT3A, IGF2R and POU5F1 between blastocysts produced in vivo and in vitro. However, a significantly lower amount of DNMT1 transcripts was found in the in vitro cultured embryos (P < 0.05) compared with their in vivo derived counterparts. In conclusion, this study reported only minor changes in the expression of developmentally important genes and satellite DNA methylation related to the in vitro embryo production system.
体内和体外产生的牛胚胎在分子特征方面存在差异，包括表观遗传标记和基因表达谱。本研究调查了牛睾丸卫星I（BTS）和牛α卫星I（BTαS）DNA序列中的CpG甲基化状态，并同时研究了体外或体内产生的印度瘤牛胚胎中与DNA甲基化（DNMT1和DNMT3A）、生长发育（IGF2R）和多能性（POU5F1）密切相关的转录本的相对丰度。结果显示，与体内产生的胚胎相比，体外产生的胚胎中BTS的甲基化程度更高（P < 0.05），而两组中BTαS的甲基化状态相似。体内和体外产生的囊胚之间，DNMT3A、IGF2R和POU5F1的转录本丰度没有显著差异。然而，与体内来源的胚胎相比，体外培养的胚胎中DNMT1转录本的数量显著减少（P < 0.05）。总之，本研究报告了与体外胚胎生产系统相关的发育重要基因表达和卫星DNA甲基化的微小变化。
