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胆囊癌中CD8炎性细胞与E-钙黏蛋白表达的负相关：组织芯片与影像学分析

Inverse correlation between CD8 inflammatory cells and E-cadherin expression in gallbladder cancer: Tissue microarray and imaging analysis.

作者信息

Kai Keita, Masuda Masanori, Aishima Shinichi

机构信息

Keita Kai, Shinichi Aishima, Department of Pathology, Saga University Hospital, Saga 849-8501, Japan.

出版信息

World J Clin Cases. 2017 Jan 16;5(1):1-8. doi: 10.12998/wjcc.v5.i1.1.

DOI:10.12998/wjcc.v5.i1.1
PMID:28138440
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5237822/
Abstract

AIM

To investigated the association between the tumor cells' expression of E-cadherin and the numbers of several types of inflammatory cells infiltrating into the invasive portion of gallbladder cancer (GBC).

METHODS

We analyzed 50 GBC cases for which a sufficient amount of tumor tissues for tissue microarray (TMA) had been saved. Three tissue cores (3.0 mm) of invasive lesion from each case were used for the TMA. The 4-μm cut sections on slides were immunostained using primary antibodies including E-cadherin for cancer cells, leukocyte common antigen for leukocyte, myeloperoxidase for neutrophils, CD3 for T cells, CD4 for helper T cells, CD8 for killer T cells, CD20 for B cells and CD68 for macrophages. The immunostained slides were digitally analyzed by imaging analysis software.

RESULTS

A significant inverse correlation between the number of infiltrating CD8 cells at invasive areas and the expression of E-cadherin by cancer cells was observed ( = 0.0001), although the degree of this correlation was relatively weak ( = 0.32). The number of CD8 cells and the cancer cells' E-cadherin expression were also significantly correlated with tumor differentiation (well-differentiated poorly differentiated) ( = 0.0467 and = 0.0294, respectively). Inverse correlation of T-stage and the number of CD8 cell infiltration was observed with statistical significance in comparison of T2 and T3 cases ( = 0.0324).

CONCLUSION

Our findings indicate an inverse correlation of CD8 T cell infiltration and cancer cells' E-cadherin expression at invasive areas of GBC. Further analyses are essential to test these findings.

摘要

目的

研究胆囊癌（GBC）浸润部位肿瘤细胞E-钙黏蛋白的表达与几种炎性细胞浸润数量之间的关系。

方法

我们分析了50例保存有足够数量用于组织芯片（TMA）的肿瘤组织的GBC病例。从每个病例的浸润性病变中取3个组织芯（3.0毫米）用于TMA。玻片上4微米厚的切片使用包括针对癌细胞的E-钙黏蛋白、针对白细胞的白细胞共同抗原、针对中性粒细胞的髓过氧化物酶、针对T细胞的CD3、针对辅助性T细胞的CD4、针对杀伤性T细胞的CD8、针对B细胞的CD20以及针对巨噬细胞的CD68的一抗进行免疫染色。通过成像分析软件对免疫染色的玻片进行数字分析。

结果

尽管这种相关性的程度相对较弱（r = 0.32），但在浸润区域观察到浸润性CD8细胞数量与癌细胞E-钙黏蛋白表达之间存在显著的负相关（r = 0.0001）。CD8细胞数量和癌细胞E-钙黏蛋白表达也与肿瘤分化（高分化与低分化）显著相关（分别为r = 0.0467和r = 0.0294）。在比较T2和T3病例时，观察到T分期与CD8细胞浸润数量呈负相关，具有统计学意义（r = 0.0324）。

结论

我们的研究结果表明，在GBC浸润区域，CD8 T细胞浸润与癌细胞E-钙黏蛋白表达呈负相关。进一步分析对于验证这些发现至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/191489c50cd8/WJCC-5-1-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/41a24c86c392/WJCC-5-1-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/c727f7c5aa1d/WJCC-5-1-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/9b7a9508c1ab/WJCC-5-1-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/888018cd0a8c/WJCC-5-1-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/794716e9cc39/WJCC-5-1-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/6751737dbc21/WJCC-5-1-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/191489c50cd8/WJCC-5-1-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/41a24c86c392/WJCC-5-1-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/c727f7c5aa1d/WJCC-5-1-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/9b7a9508c1ab/WJCC-5-1-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/888018cd0a8c/WJCC-5-1-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/794716e9cc39/WJCC-5-1-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/6751737dbc21/WJCC-5-1-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec0c/5237822/191489c50cd8/WJCC-5-1-g007.jpg

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