[Bacteria detected in the duodenal aspirate of patients with biliary tract disorders].

In 614 patients with pains in the right hypochondrium the cyto-bacterioscopy and bile culture were performed. In 575 patients bile samples were obtained by sterile catheterization and in 39 patients by biliary tract puncture during surgical intervention. It resulted that for a reliable cyto-bacterioscopic examination and its agreement with bile culture results it is necessary to investigate only the samples with pH greater than or equal to 5 and during the first two hours after their collection. The isolated bacterial species can be considered to be etiologic agents of the biliary infection depending on the following parameters: significant quantitative threshold of minimum 10(5) colony forming units per milliliter, presence of inflammatory reaction and activity of the gastric acid barrier.