González-Sarrías Antonio, Núñez-Sánchez María Ángeles, Tomás-Barberán Francisco A, Espín Juan Carlos
Research Group on Quality, Safety, and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC , P.O. Box 164, 30100 Campus de Espinardo, Murcia, Spain.
J Agric Food Chem. 2017 Feb 1;65(4):752-758. doi: 10.1021/acs.jafc.6b04538. Epub 2016 Dec 2.
Oxidative stress is involved in cell death in neurodegenerative diseases. Dietary polyphenols can exert health benefits, but their direct effects on neuronal cells are debatable because most phenolics are metabolized and do not reach the brain as they occur in the dietary sources. Herein, we evaluate the effects of a panel of bioavailable polyphenols and derived metabolites at physiologically relevant conditions against HO-induced apoptosis in human neuroblastoma SH-SY5Y cells. Among the 19 metabolites tested, 3,4-dihydroxyphenylpropionic acid, 3,4-dihydroxyphenylacetic acid, gallic acid, ellagic acid, and urolithins prevented neuronal apoptosis via attenuation of ROS levels, increased REDOX activity, and decreased oxidative stress-induced apoptosis by preventing the caspase-3 activation via the mitochondrial apoptotic pathway in SH-SY5Y cells. This suggests that dietary sources containing the polyphenol precursors of these molecules such as cocoa, berries, walnuts, and tea could be potential functional foods to reduce oxidative stress associated with the onset and progress of neurodegenerative diseases.
氧化应激参与神经退行性疾病中的细胞死亡。膳食多酚具有健康益处，但其对神经元细胞的直接作用仍存在争议，因为大多数酚类物质会被代谢，无法以膳食来源中的形式进入大脑。在此，我们评估了一组生物可利用的多酚及其衍生代谢物在生理相关条件下对人神经母细胞瘤SH-SY5Y细胞中过氧化氢（HO）诱导的细胞凋亡的影响。在所测试的19种代谢物中，3,4-二羟基苯丙酸、3,4-二羟基苯乙酸、没食子酸、鞣花酸和尿石素通过降低活性氧水平、增强氧化还原活性以及通过线粒体凋亡途径阻止半胱天冬酶-3激活来预防氧化应激诱导的细胞凋亡，从而防止神经元凋亡。这表明，含有这些分子的多酚前体的膳食来源，如可可、浆果、核桃和茶，可能是潜在的功能性食品，可减少与神经退行性疾病的发生和进展相关的氧化应激。
